FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1476989
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCAGCTCTCAGACTGTTCATTTCCAAATAAA26060.17644004119191137No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20000.13541062255710773No Hit
GTATAATAACTGGCCTCCGTCCGCGCTCTTCGGCCCTGGGACCAAAGTGG18940.128233859561581No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17580.11902593722769769No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17230.11665625133294832No Hit
CTCCAGACCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCCAGAATTTC17030.11530214510737724No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA16510.11178146892089243No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16020.1084639086682433No Hit
GTGTATTACTGTGCGAAAGTGGAGGGTTATTATGGTTCGGGAGAGCTTGA15040.10182878816294501No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)