FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1425388
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC40710.2856064454029359No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCAGATGGTTACCAGTGATGTCGCGGGAA37870.265682045871019No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCAGATGGTTACCA34510.2421095168473426No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT33460.23474310152744376No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC32690.2293410636261846No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG30970.21727417376882643No Hit
AGCCAAGACAGGCCCCTGTTCGTGTCATCTTTGGTAAAAACAATCGGCCC29090.2040847825293885No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT28620.200787434719529No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT27800.19503461513636988No Hit
GTAATATATGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGAACTGGTTCTGGGACGTGTCTACTGA27020.18956242089873074No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC25160.17651334233205274No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG24280.1703395847306137No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG24260.17019927205785373No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC24090.1690066143393939No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC23970.16816473830283402No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT23340.16374488911089471No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG22710.1593250399189554No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG21790.1528706569719964No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG20930.14683721204331734No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC20920.14676705570693735No Hit
GTCTTGGCTTCTGCTGGTACCAGGTTCCAAAAAAGTATCTGAGGCTGTCTCCTTGGCATGTGATCCTGACTGT20870.1464162740250374No Hit
ACTCAGGACCCTGCTGTGTCTGTGCCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGATA20370.14290845720603793No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20240.14199642483309807No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGCCCTTGGGA20100.14101423612377822No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC19870.13940064038703848No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG19540.13708548128649883No Hit
GTCTGTGCCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGATACTTTTTTGGAACCTGGTA19340.13568235455889904No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT19230.13491063485871918No Hit
CTACAATAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAGCTGTGGTTCCTGAGCTGGAG18580.1303504729940199No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18050.12663218716588046No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCT17600.12347515202878094No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17530.12298405767412102No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17360.12179139995566118No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16800.1178626451183818No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG16770.11765217610924184No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16690.11709092541820192No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16580.11631920571802204No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16450.11540717334508217No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16280.11421451562662237No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT16120.11309201424454253No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16110.11302185790816255No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15940.11182920018970273No Hit
CTATTATTGTAGTTCCCGCGACATCACTGGTAACCATCTGATATTCGGCG15860.11126794949866281No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGA15770.11063654247124292No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT15700.110145448116583No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG15370.10783028901604336No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCAGATGGTTACCAG14970.10502403556084379No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14760.10355075249686402No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14560.10214762576926423No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA14530.10193715676012426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC2800.046.741183
GTATCAA20200.044.354731
GGTATCA7400.042.376751
CTTATAC3100.042.241551
TTATACA3100.042.2178422
ATCGATT350.001325914239.5608543
TATCAAC22650.039.5347752
CGTAGAG3250.039.23713728
ACGTAGA3450.036.95733327
AGACGTA3550.035.9074625
GACGTAG3600.035.41495526
ACGAGAC3600.035.40128722
TACACAT3900.035.3239675
ATCAACG25450.035.1851733
TCAACGC25950.034.639954
TATCGAT400.002558661434.6035442
CAACGCA26200.034.046515
ATACACA4250.034.0356834
AACGCAG27200.032.79486
AGGGGGG2950.031.34690770
Produced by FastQC (version 0.11.5)