FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1425388
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC41110.2884126988581355No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCAGATGGTTACCAGTGATGTCGCGGGAA33230.233129505790704No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT32260.22632434116184505No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC32150.22555262146166516No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCAGATGGTTACCA31390.22022073989678598No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT31200.2188877695055662No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT30060.21088994715824744No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG29430.2064700979663081No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29220.20499681490232835No Hit
AGCCAAGACAGGCCCCTGTTCGTGTCATCTTTGGTAAAAACAATCGGCCC28250.19819165027346938No Hit
GTAATATATGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGAACTGGTTCTGGGACGTGTCTACTGA26480.1857739787342113No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG24460.1716023987854535No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG24160.16949770869405384No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT23880.16753333127541414No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23080.161920824365015No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC22670.15904441457343543No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC21870.1534319076630363No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGCCCTTGGGA21820.15308112598113635No Hit
GTCTTGGCTTCTGCTGGTACCAGGTTCCAAAAAAGTATCTGAGGCTGTCTCCTTGGCATGTGATCCTGACTGT21490.1507659668805967No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG21400.15013455985317684No Hit
GTCTGTGCCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGATACTTTTTTGGAACCTGGTA20590.1444518966063977No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG20150.14136501780567817No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC20060.14073361077825827No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19880.13947079672341847No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG19810.13897970236875853No Hit
ACTCAGGACCCTGCTGTGTCTGTGCCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGATA19420.13624360524993898No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18810.1319640687307596No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC18490.12971906596659996No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG18470.12957875329384No Hit
CTACAATAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAGCTGTGGTTCCTGAGCTGGAG18430.12929812794832005No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT18300.12838609557538017No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA17470.12256311965584109No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCT17380.12193171262842117No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG17180.12052858590082138No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17130.12017780421892145No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT16810.1179328014547618No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15940.11182920018970273No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15790.11077685514400289No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15780.1107066988076229No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15680.11000513544382302No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT15380.10790044535242332No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGA15320.1074795073341434No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15260.10705856931586347No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT14920.10467325387894383No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14840.10411200318790394No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14740.10341043982410403No Hit
GCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAGCTGTGGTTCCTGAGCTGGAGCCAGAGAATCGGT14690.1030596581422041No Hit
CTATTATTGTAGTTCCCGCGACATCACTGGTAACCATCTGATATTCGGCG14660.10284918913306411No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT14430.10123559339632437No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14330.10053403003252447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACGC453.0931915E-853.5243234
GGTATCA8700.043.1170541
GTATCAA21750.043.037941
ATAAACG602.972538E-740.143243
TATCAAC25000.037.2988052
ATCAACG27200.034.281993
TCAACGC27100.034.1545564
GTGGTAT3350.033.900881
AACGCAG28350.033.5017436
CAACGCA27750.033.4785355
GTCTAGG652.3616143E-531.767261
TGGTATC3500.031.459192
AGAGTAC30450.031.32407211
CAGAGTA31200.030.57108710
CGCAGAG31400.030.264588
GTATTGT605.502125E-428.6787741
TACGGGT2800.028.27701415
ACGCAGA34200.027.680277
AGTACGG33300.026.7818913
TATGCTT1258.5527654E-426.5920770
Produced by FastQC (version 0.11.5)