FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757118

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757118
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1276607
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16100.12611555474785896No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA45800.042.9748341
GGTATCA17700.039.7144361
TCAACGC53000.036.8060464
ATCAACG53650.036.4879233
CAACGCA53550.036.428025
TATCAAC54050.036.0910342
AACGCAG55400.035.2170756
ACGCAGA58200.033.4259037
CGCAGAG58550.033.1140868
AGAGTAC59800.032.1948911
CAGAGTA61500.031.41532110
GCAGAGT62500.031.0773989
CACGCGT605.047656E-429.10404250
GAGTACG47850.028.04271712
AGTACGG48100.027.9683113
TAGCGAA756.389781E-527.45460715
GTGGTAT7250.026.0131231
ACGCGTG700.001237754624.96126251
TGGTATC7450.024.8496482
GTACGGG53600.024.77829414
Produced by FastQC (version 0.11.5)