FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757121

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757121
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219977
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT3320.15092486941816646No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGG503.6248474E-451.4356370
AGATACG306.674105E-445.4649328
GTATATA306.764678E-445.3407671
CGGGCTT306.802703E-445.2892317
GTGATCG408.9290173E-442.85072769
GTATCAA11550.039.1579321
ACGGGCT702.4954716E-838.8193416
GGTATCA4450.038.208511
TCGTATG655.2304495E-737.3264640
CTCGTAT655.2974065E-737.26595339
ACGCGTA655.98764E-736.68818727
CTTATAC656.0737693E-736.621391
TATCAAC12850.035.164422
CGTATGC601.2711935E-534.7570441
GTAGTAA701.0261792E-634.24719231
TCAACGC13300.034.2300954
CGCGTAG701.0616714E-634.098728
AGACGCG807.955714E-834.0288125
ACGAGAC701.0904387E-633.98237622
CAACGCA13400.033.9746485
Produced by FastQC (version 0.11.5)