FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences920288
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30550.331961299071595No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA27040.29382106471017766No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20890.22699415835042946No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT19230.20895632671511527No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19000.20645710907889703No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC18890.20526183107896656No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18230.19809016307938385No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18030.19591693035223753No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17450.18961455544351333No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17300.18798463089815362No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG16440.1786397301714246No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15680.17038144580826872No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15330.16657828853576273No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15260.16581765708126153No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAGATAGTA14930.16223182308147016No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG14800.16081922180882507No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14780.16060189853611045No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCAGGGGCCCTGGT14730.16005859035432385No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTTCTTGGCCTGGTA14550.1581026808998922No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTTCTTGGCCTGGTACCAGCAGAAACCTGGCCAGACTCCCAGGCT14430.15679874126360444No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCACCCTGCAGTCTGAAGATT14120.15343023053652768No Hit
GAGTTCACTCTCACCATCAGCACCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCACCAATATAATAACTGG14060.1527782607183838No Hit
CATCTATGGTGCATCCACCAGGGCCCCTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA14020.15234361417295456No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT13790.1498443965367363No Hit
ATATAATAACTGGCCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT13550.1472365172641608No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13360.1451719461733718No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG13340.1449546229006572No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCCGTTGCTAC13300.14451997635522792No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12930.14049949581000729No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA12910.14028217253729267No Hit
GTTATTATATTGGTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGGTGCTGATGGTGAGAGTGAACT12840.13952154108279147No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12700.13800027817378907No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG12590.13680500017385863No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11920.12952467053791858No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCAACGGCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGG11920.12952467053791858No Hit
GGCCAAAAGTGTACGGTGGCCAGTTATTATATTGGTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGGTG11890.12919868562884662No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC11370.12354828053826629No Hit
CTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCCCTGGTGTCCCA11250.12224434090197853No Hit
CCATAGATGAGGAGCCTGGGAGTCTGGCCAGGTTTCTGCTGGTACCAGGC11210.12180969435654926No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC11200.12170103272019193No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT11110.1207230779929761No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA11100.1206144163566188No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT11060.12017976981118954No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10940.11887583017490178No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG10900.11844118362947251No Hit
GTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCT10860.11800653708404325No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10760.11691992072047012No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10740.11670259744775548No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10720.11648527417504086No Hit
GGGCTGGAGTGGGTGGCAGTGACATCATTTGATGGAAGTAATAAAGATTA10590.11507267290239577No Hit
ATAATATATAGCCGTGTCCTCAATTCTCAGGCTGTTCATTTGCAGAAACA10570.11485534962968115No Hit
GCACTAGAAGTCGGCGGTGTTTCCATTCGGTGATCAGCACCGAACACAGA10470.113768733266108No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT10340.11235613199346291No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC10140.11018289926631664No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCA9850.10703171181195453No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA9730.10572777217566676No Hit
GTGTTAGCAGCTTCTTGGCCTGGTACCAGCAGAAACCTGGCCAGACTCCCAGGCTCCTCATCTATGGTGCATCCA9660.10496714072116556No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9640.10474981744845091No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGA9640.10474981744845091No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9590.10420650926666435No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG9470.10290256963037658No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT9330.10138130672137419No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9280.1008379985395876No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9240.10040335199415835No Hit
CCACTGGAGAGATAGTACTGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTC9220.10018602872144372No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGAT200.006710103252.97139466
CAGACCG400.00930550948.7624570
GGTATCA6900.043.4978751
ACGGGTA803.45608E-1143.09712216
GTATCAA18650.042.1748851
TATCAAC21800.035.742932
ATCAACG22200.034.943613
CATACTA400.00249514234.78005252
TCAACGC22350.034.709094
TATCTCG709.646428E-734.53962736
CAACGCA22950.033.8016625
AACGCAG23250.033.51386
TCGTATG751.6321956E-632.2914740
CGGGTGA652.3313056E-531.8255676
CGCAGAG24600.031.8165118
AGAGTAC24800.031.6989611
TTATACA1207.8216544E-1131.6045552
TATACAC1207.8216544E-1131.6045553
GCAGAGT25450.030.8893599
GTGTATA902.0048356E-730.64850613
Produced by FastQC (version 0.11.5)