FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757124

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757124
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences920288
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31340.34054556834382277No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT25250.27437063180221843No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA24180.2627438367119858No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21460.23318787162279636No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18760.2038492298063215No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18030.19591693035223753No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17740.19276574289787546No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC17700.1923310963524462No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17130.1861373830800793No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15330.16657828853576273No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14510.15766803435446297No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14420.15669007962724713No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAGATAGTA14150.15375621544559964No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCACCCTGCAGTCTGAAGATT14050.1526695990820265No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCAGGGGCCCTGGT13940.15147432108209602No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13850.1504963663548802No Hit
CATCTATGGTGCATCCACCAGGGCCCCTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA13720.14908376508223514No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTTCTTGGCCTGGTACCAGCAGAAACCTGGCCAGACTCCCAGGCT13620.14799714871866199No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13500.1466932090823742No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT13170.14310737508258287No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT13140.1427813901735109No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13090.14223808199172433No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCCGTTGCTAC12910.14028217253729267No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12890.14006484926457805No Hit
ATATAATAACTGGCCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT12870.1398475259918634No Hit
GTTATTATATTGGTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGGTGCTGATGGTGAGAGTGAACT12800.13908689453736223No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTTCTTGGCCTGGTA12770.13876090962829027No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG12740.13843492471921834No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG12720.1382176014465037No Hit
GAGTTCACTCTCACCATCAGCACCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCACCAATATAATAACTGG12470.13550106053757086No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG12330.13397979762856843No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12120.13169790326506484No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA11730.1274600994471296No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11690.12702545290170034No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA11610.12615615981084183No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCAACGGCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGG11570.12572151326541256No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11390.12376560381098091No Hit
GGCCAAAAGTGTACGGTGGCCAGTTATTATATTGGTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGGTG11150.12115772453840537No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT11090.12050575472026148No Hit
GTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCT11070.12028843144754686No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT10980.11931047672033102No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10910.11854984526582983No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC10900.11844118362947251No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA10740.11670259744775548No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT10680.11605062762961159No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10660.11583330435689697No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC10430.11333408672067875No Hit
GCACTAGAAGTCGGCGGTGTTTCCATTCGGTGATCAGCACCGAACACAGA10410.1131167634479641No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA10330.11224747035710561No Hit
ATAATATATAGCCGTGTCCTCAATTCTCAGGCTGTTCATTTGCAGAAACA10200.11083486908446051No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10110.10985691435724469No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT10100.10974825272088737No Hit
GGGCTGGAGTGGGTGGCAGTGACATCATTTGATGGAAGTAATAAAGATTA10030.10898762126638618No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA10020.10887895963002886No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9960.10822698981188497No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9930.10790100490281303No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9890.10746635835738377No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9780.10627108035745332No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9780.10627108035745332No Hit
CTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCCCTGGTGTCCCA9600.10431517090302167No Hit
GTACTGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGC9540.10366320108487778No Hit
GTGTTAGCAGCTTCTTGGCCTGGTACCAGCAGAAACCTGGCCAGACTCCCAGGCTCCTCATCTATGGTGCATCCA9470.10290256963037658No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9420.10235926144859002No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC9380.10192461490316075No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9340.10148996835773148No Hit
CCACTGGAGAGATAGTACTGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTC9310.10116398344865954No Hit
CCATAGATGAGGAGCCTGGGAGTCTGGCCAGGTTTCTGCTGGTACCAGGC9220.10018602872144372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7750.047.1071621
GTATCAA18850.039.2834741
AGTTAGT450.001698695737.613369
TATCAAC21150.034.680172
AAGGTCG400.002611467834.4584115
TAGTACG501.8815679E-434.435944
TCAACGC21800.033.6461224
ATCAACG21950.033.41623
CAACGCA22200.032.8847735
AACGCAG22450.032.5185746
AATGGTA1701.5746627E-731.49812770
CGCAGAG23850.030.9186558
AGAGTAC23750.030.9037511
TTCGGGG8600.030.3535770
GCAGAGT25050.029.4375219
ACGCAGA26050.028.170617
CAGAGTA26100.028.12123110
CAGTTCG12350.027.64056270
CGTCCAT500.007779788727.56672510
TGTTAGA5100.027.56086270
Produced by FastQC (version 0.11.5)