FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1283431
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATACCTGGCCTCAGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT45210.35225890601052956No Hit
GTAATACACGGCCGTGTCCGCGGCAGTCACAGAGTTCAGCCTCAGGGAGA35940.2800306366294721No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA30700.2392025749728657No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26310.2049973859132279No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT26040.2028936499118379No Hit
CCATAGATGAGGGCCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC25330.19736160338966413No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT24960.19447870590627778No Hit
GTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTC24950.19440078975807817No Hit
GTCTCTGGTGACTCCATCGGCACTGGTGGTAACTTCTGGAGCTGGATCCG24030.18723250412371215No Hit
GTGTATTACTGTGCGAGATCCCAGGGACGTACGTTTGGGGGAGTTGTCGT23860.18590792960431843No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT22420.17468800426357162No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22120.1723505198175827No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA20540.1600397684020411No Hit
GTAGTAGGTGGTCTCACTGTAATAGATGGACCCAATCCACTCCAGGCCCTTCCCTGGGTGCTGGCGGATCCAGCT19850.1546635541762666No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC19220.14975483683968988No Hit
ACCTACTACAGCCCGTCCCTCAAGAGTCGGGCTACCATTTCAGACGACACGTCTAAGAACCAGTTCTCCCTGAGG17550.13674284009035156No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC17350.13518451712635896No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC17160.13370411031056598No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16790.13082121282717965No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16730.13035371593798187No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16620.1294966383077859No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16570.12910705756678778No Hit
GTAATAGATGGACCCAATCCACTCCAGGCCCTTCCCTGGGTGCTGGCGGA16540.1288733091221889No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16350.1273929023063959No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG16310.1270812377135974No Hit
CACCTGCACTGTCTCTGGTGACTCCATCGGCACTGGTGGTAACTTCTGGA15900.12388667563741254No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15860.12357501104461402No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC15520.12092586200582657No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT15040.11718588689224431No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA14820.11547173163185243No Hit
CAGTAATACACGGCCGTGTCCGCGGCAGTCACAGAGTTCAGCCTCAGGGAGAACTGGTTCTTAGACGTGTCGT14740.1148484024462554No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14650.11414715711245871No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGTGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAACAG14420.11235508570386721No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14420.11235508570386721No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13970.10884885903488385No Hit
GGATACCAGTGGCCCTGGTGGATGCACCATAGATGAGGGCCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGG13780.10736845221909086No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT13490.10510888392130156No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATACCTGG13190.10277139947531266No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT13130.10230390258611488No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAACAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGGC13050.10168057340051782No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)