FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757129
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1158618
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20930.18064625269070567No Hit
GTCTCTGGTGCCTCCATCAGCAGTGATGATTACTACTGGAGTTGGATCCG16110.1390449656400988No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCGGCTCAGCTCCTGG15540.13412531136232994No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15190.13110447101633152No Hit
GTCTACGGTGACTGCGGTCCTTGACCACTGGGGCCAGGGAACCCTGGTCACCGTCTCTTCAACCTCCACCAAGGG15090.1302413737746177No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14970.1292056570845611No Hit
GTAGGAGACAGAGTCACCATCACCTGCCAGGCGAGTCACGACATTAGCAACAGTTTAAATTGGTATCAGCAGAAA14620.12618481673856266No Hit
CACCTGCACTGTCTCTGGTGCCTCCATCAGCAGTGATGATTACTACTGGA14290.12333659584090702No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14250.12299135694422147No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACCTGCCAGGCGAGTCACGACATTAGCAA14100.12169671108165073No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14100.12169671108165073No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13790.11902110963233783No Hit
ACTATAATCCGTCCCTCAAGAGTCGACTTGCCATCTCACTAGACACGTCC13780.11893479990816645No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC13680.11807170266645263No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG13200.11392883590622621No Hit
GTATGATGATCGGCCCTCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT13180.11375621645788345No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13060.11272049976782685No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13020.1123752608711413No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12590.10866394273177182No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG12280.10598834128245893No Hit
CCGTAGACAGGGTGGCACAGAAATAGACGGCCGTGTCTGCGGCAGTCACG12070.10417583707485988No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11910.10279488148811775No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11670.10072344810800454No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT200.007335525551.7919212
GTATCAA17650.034.6511841
TACCGCT450.00453197530.79117443
TCAACGC19900.030.5359634
TATCAAC20300.030.2744312
CAACGCA20400.029.9567785
AACGCAG21250.028.7585076
GTGGTAT3150.027.4232521
CAGCGCT3550.026.2595584
AGAGTAC23150.026.25018911
CGCAGAG23550.025.8032118
CAGAGTA24150.025.59103610
ACGCAGA24000.025.4621647
TGTTAGA5550.025.31508670
TTCGGGG8700.024.95797570
AGCGCTG3700.024.2618335
GCAGAGT25900.023.59539
GAGTACG21700.021.95786712
AGTACGG21950.021.70777313
ATCAACG28900.021.0264933
Produced by FastQC (version 0.11.5)