FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1657952
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC55080.33221709675551525No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC49850.3006721545617726No Hit
GCCTTGGGCTGACCTAGGACGGTGAGCTTGGTCCCTCCGCCGAAGACCAC46100.2780538881704657No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT42390.2556768832873328No Hit
GATTATTACTGTGAAGCATGGGATGACAGCCTGAATGGCGTGGTCTTCGG39800.24005520063307018No Hit
ACCTAGGACGGTGAGCTTGGTCCCTCCGCCGAAGACCACGCCATTCAGGC34590.20863088919341452No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC33160.2000057902761962No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG32070.19343141417845633No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCCGCGTCTGAGACCC32010.19306952191619542No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG31880.19228542201463011No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTGAGCTTGGTCCCTC30650.18486663063828143No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG30500.18396189998262918No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT29870.18016203122888963No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG29740.17937793132732432No Hit
GACCTAGGACGGTGAGCTTGGTCCCTCCGCCGAAGACCACGCCATTCAGG29350.1770256316226284No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG28010.16894337109880142No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCCGCGTCT26580.16031827218158307No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG25510.15386452683793017No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC25330.15277885005114744No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGAAGCATGGGATGACAGCCTGAATGGCGTGGTCTTCGGCGGAGGGAC24300.1465663662156685No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA24150.1456616355600162No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23390.14107766690471135No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC23300.14053482851132No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT23140.13956978247862423No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG22630.1364936982494065No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT22500.1357095983478412No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC22460.13546833683966725No Hit
CCCCTGAGGGCCGCTGATTATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGATCTGTTGGTACCAGTTTA22360.1348651830692324No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21800.13148752195479727No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC21510.12973837602053617No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21390.12901459149601435No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA21010.12672260716836192No Hit
GTGCTGACTCAGCCACCCTCCGCGTCTGAGACCCCCGGGCAGAGGGTCAC20930.12624008415201407No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG20550.12394809982436163No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20290.12237990002123102No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC19880.11990696956244813No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19840.11966570805427418No Hit
GTCTTCGGCGGAGGGACCAAGCTCACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC19210.11586583930053462No Hit
GTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG19190.11574520854644767No Hit
GATTATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGATCTGTTGG19150.11550394703827371No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA19150.11550394703827371No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT19070.11502142402192585No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT18360.11073903225183841No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17740.10699947887514234No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA16670.10054573353148945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34300.043.114291
GGTATCA13050.041.9995541
TATCAAC38550.038.2485922
TCAACGC41150.036.166794
ATCAACG41250.036.0791133
CAACGCA42050.035.4746325
AACGCAG43050.034.65066
ACGCAGA46600.032.083877
AGAGTAC47600.031.55648811
CGCAGAG47950.031.1096638
TATACGG450.004655834830.6227025
CAGAGTA49500.030.2756310
GCAGAGT52350.028.7590089
AGTACGG44000.026.46507613
GAGTACG44900.026.01132612
TGTGTCG2601.574881E-824.35018570
GTGGTAT4850.023.4547211
GTACGGG51250.022.72123714
TACGGGG21450.022.64649415
GAGTACT14800.022.3469712
Produced by FastQC (version 0.11.5)