FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1657952
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC50760.30616085387272973No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC50160.30254193125012063No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCCGCGTCTGAGACCC43850.26448292833568166No Hit
GATTATTACTGTGAAGCATGGGATGACAGCCTGAATGGCGTGGTCTTCGG39730.2396329929937658No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT38580.23269672463376503No Hit
GCCTTGGGCTGACCTAGGACGGTGAGCTTGGTCCCTCCGCCGAAGACCAC35500.21411958850437166No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCCGCGTCT33730.2034437667676748No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC32250.19451709096523906No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG32130.19379330644071724No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG30910.18643483044141204No Hit
GTGCTGACTCAGCCACCCTCCGCGTCTGAGACCCCCGGGCAGAGGGTCAC29720.17925730057323733No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT29520.17805099303236763No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG29120.17563837795062825No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27110.16351498716488777No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG26890.16218804886993107No Hit
ACCTAGGACGGTGAGCTTGGTCCCTCCGCCGAAGACCACGCCATTCAGGC25480.15368358070679972No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC24750.1492805581826253No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGAAGCATGGGATGACAGCCTGAATGGCGTGGTCTTCGGCGGAGGGAC24680.14885835054332092No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTGAGCTTGGTCCCTC24570.14819488139584258No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC23300.14053482851132No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23010.13878568257705892No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA22860.13788095192140665No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG22330.13468423693810194No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22080.13317635251201482No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21980.13257319874157997No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG21870.13190972959410163No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT21280.12835112234853602No Hit
CCCCTGAGGGCCGCTGATTATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGATCTGTTGGTACCAGTTTA21260.12823049159444905No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG21130.12744639169288374No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT20930.12624008415201407No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA20780.12533535349636177No Hit
GTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG20570.1240687305784486No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20000.12063075408696995No Hit
GACCTAGGACGGTGAGCTTGGTCCCTCCGCCGAAGACCACGCCATTCAGG19750.11912286966088283No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT19680.11870066202157843No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT19130.11538331628418676No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA19080.11508173939896932No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC18690.11272943969427342No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT18420.11110092451409932No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA18200.10977398621914265No Hit
GATTATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGATCTGTTGG18000.10856767867827295No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT17770.10718042500627278No Hit
GTCTTCGGCGGAGGGACCAAGCTCACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC17500.1055519098260987No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG17460.10531064831792478No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17290.10428528690818552No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGAAG16810.10139014881009824No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG16730.10090762579375036No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37300.043.9343261
GGTATCA13100.043.6257251
TATCAAC40700.039.9161532
TCAACGC43100.037.1344264
ATCAACG43650.036.824233
CAACGCA43900.036.6145255
AACGCAG45100.035.7950976
CGTCCGA400.00261853734.44103622
ACGCAGA48650.033.263917
CGCAGAG49000.032.9580278
AGAGTAC50050.032.40517411
CAGAGTA51650.031.40133710
GCAGAGT53300.030.429259
GTGGTAT5200.029.1309851
AGTACGG46400.028.57197813
GAGTACG47550.028.17063512
TACGGGG24000.026.54349115
TGGTATC5600.026.4290472
GTACGGG54200.024.3966114
ACGGGGG10450.021.08734716
Produced by FastQC (version 0.11.5)