FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences929221
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG31940.3437287792677953No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAACGGCCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTC26560.28583081957898065No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA21920.23589651977301418No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC21090.226964306661171No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC19530.21017605069192363No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGGCCGTTC19440.20920749746292863No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGTCAACAAGG19130.20587136967416794No Hit
CCCAATGGCCGTTCAGGCTGTCATCCCATGCTGCACAGTAATAATCAGCC17700.19048213503569117No Hit
CCTCTGAGGAGCTTCAAGTCAACAAGGCCACACTGGTGTGTCTCATAAGT16660.17928996438952627No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC16320.1756309855244339No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT15990.1720796236847854No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG15580.1676673256415858No Hit
GCCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC14960.1609950700640644No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC14700.15819702740252317No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT14330.15421519746109913No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14240.1532466442321041No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG14030.15098668669778234No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC13880.14937243131612393No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGTCAACAAGGCCACACTGGTGT13560.14592868650191937No Hit
GTAATACACAGCCGTGTCCTCGGGAGTCACAGAGTTCAACTGCAGGGAGA13310.14323826086582203No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC13160.14162400548416362No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC13080.14076306928061247No Hit
CCCTTGAGGGCCGCTGATTATTACTATAAATGAGGAGTTTGGGGGCCGTTCCTGGGAACTGCTGGTACCAGTTTA13000.1399021330770613No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA12510.13462889883031057No Hit
GCATGGGATGACAGCCTGAACGGCCATTGGGTGTTCGGCGGAGGGACCAA12260.13193847319421323No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGA12100.13021660078711092No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA11490.12365196223503344No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11080.1192396641918338No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11060.11902443014094602No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG11030.11870157906461434No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG11020.11859396203917044No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11010.11848634501372655No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC10770.11590353640307312No Hit
CCTCAAGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT10700.11515021722496585No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC10690.11504260019952198No Hit
CACCAGTGTGGCCTTGTTGACTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10640.11450451507230248No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGACTTGAAGCTCCTCAGAGGA10320.11106077025809791No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC10280.11063030215632233No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC10190.1096617489273273No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10000.10761702544389333No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGGCCGTTCA9980.10740179139300554No Hit
ATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCAC9630.10363519550246929No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC9320.10029906771370858No Hit
GTTTGGGGGCCGTTCCTGGGAACTGCTGGTACCAGTTTACAGTATTTCTT9320.10029906771370858No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT9310.10019145068826468No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGCG200.00685460252.68684464
GTATCAA19700.039.758681
GGTATCA7950.039.4954071
TGGTATC3100.035.594272
TATCAAC22450.034.5587462
TGGTTTG7200.033.9258370
TCAACGC23600.033.459184
ATCAACG23550.033.3837973
CAACGCA24000.032.9015245
AACGCAG25150.031.397086
ACGCAGA26000.030.3706387
CCTAGAC802.7721835E-630.1717033
CGCAGAG26500.029.9293388
AGAGTAC26850.029.53919811
GTTACTA604.906044E-429.2448263
GCAGAGT28150.028.5425439
CAGAGTA27800.028.52976410
CAATCGC500.007751910527.58704411
GTCTAGT658.649648E-426.5417141
CTGGTTT7450.025.47939169
Produced by FastQC (version 0.11.5)