FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1089186
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13450.044.319251
GTATCAA34950.042.688341
TCAACGC41000.036.369164
ATCAACG41100.036.280673
CAACGCA41950.035.6276325
TATCAAC42250.035.2931482
AACGCAG42800.035.0005386
ACGCAGA46400.032.2834977
CGCAGAG46800.032.154738
AGAGTAC47100.031.73204411
CAGAGTA48150.031.1115910
GCAGAGT50100.030.1054889
GAGTACG38350.029.5433512
AGTACGG38600.029.35200913
TACGAAA1209.4711315E-426.13053170
TACGGGG18550.025.80477715
GTACGGG43950.025.70065514
CCGTTAA2800.024.59807816
TACGGGA13700.024.13125615
AATCGTG1300.001510299624.12049170
Produced by FastQC (version 0.11.5)