FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1089186
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13600.043.4900361
GTATCAA36850.043.112651
TATCAAC43900.036.2640952
ATCAACG43850.036.1486173
TCAACGC44100.035.8657234
CAACGCA44700.035.3843045
AACGCAG45200.035.146646
GTGGTAT4900.032.9839021
ACGCAGA48300.032.902947
CGCAGAG48600.032.770618
AGAGTAC49250.032.54913711
CAGAGTA50200.031.86463710
GCAGAGT51400.030.9854419
AGTACGG41300.029.06800313
GAGTACG41700.028.87299212
TGGTATC5550.028.4987092
GTACGGG47250.025.33478714
TACGGGG20800.024.80776815
CGAATTA1251.3003591E-724.76807615
TACGGGC10200.022.93340315
Produced by FastQC (version 0.11.5)