FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757142

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757142
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1411237
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC36350.2575754462220024No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAACGGCCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTC33530.2375929769415059No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC29450.20868217032291528No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT28390.2011710293876932No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC27210.19280957061074788No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA25600.18140113956762755No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGTCAACAAGG23370.16559939967560375No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG23220.1645365023734497No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG22080.15645848287707875No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG21790.15440354809291423No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC21690.15369494989147817No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21280.1507896972655904No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC21180.15008109906415437No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGGCCGTTC20600.14597122949582528No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG19980.14157792064692182No Hit
CCTCTGAGGAGCTTCAAGTCAACAAGGCCACACTGGTGTGTCTCATAAGT19010.13470451809299216No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT18980.13449193863256137No Hit
GCCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC18690.13243700384839682No Hit
CCCTTGAGGGCCGCTGATTATTACTATAAATGAGGAGTTTGGGGGCCGTTCCTGGGAACTGCTGGTACCAGTTTA17650.12506758255346195No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGA17060.1208868531649893No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGTCAACAAGGCCACACTGGTGT16830.11925707730168639No Hit
CCCAATGGCCGTTCAGGCTGTCATCCCATGCTGCACAGTAATAATCAGCC16390.11613924521536781No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA15850.11231281492761315No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG15610.11061217924416664No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15400.10912412302115096No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14930.10579371147440154No Hit
GCATGGGATGACAGCCTGAACGGCCATTGGGTGTTCGGCGGAGGGACCAA14790.10480167399239107No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG14630.10366791687009341No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA14130.10012492586291319No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)