FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757145

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757145
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1308699
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41750.042.3927351
GGTATCA16200.041.448081
TATCAAC48850.036.1994132
TCAACGC49600.035.6520424
GTGGTAT5250.035.4176061
ATCAACG50000.035.3668253
CAACGCA50400.035.2226565
TCGTATG2200.034.6071540
AACGCAG52450.034.0427676
CGTATGC2100.032.98835441
ACGCAGA54000.032.874487
ACGGGTA3600.032.49221816
CGCAGAG56000.031.5775228
AGAGTAC55700.031.56230211
CAGAGTA56750.031.03895410
GCAGAGT58900.030.0227729
CTCGTAT2400.028.82046339
TGGTATC6800.028.3323152
GAGTACG46000.027.89677412
AGTACGG45850.027.83796913
Produced by FastQC (version 0.11.5)