FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2948803
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTATACTGCATCCAATTTGAAAAATGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAGTTCA87880.2980192301757696No Hit
GTAATACACTGCCGTGTCTGCGGCGGTCACAGAACTCAGCTGCAGGGAGA78600.26654883354364467No Hit
GTGTATTACTGTGCGAGACATTATCAGCGCCATTTTGAATACTGGGGCCA72740.24667636325654851No Hit
GTCTCTGGTGGCTCCATCAGCGATGGGACTTACTACTGGGCCTGGATCCG64250.21788501978599453No Hit
GTATAGATCAGGGACTTAGGGGCTTTTTCTGGTTTCTGCTGATACCAGGC62220.21100087052271718No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT56030.19000930207952177No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT54170.18370165792696222No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT52300.17736010170906635No Hit
GTATTACTGTGCGAGACATTATCAGCGCCATTTTGAATACTGGGGCCAGGGAATCCTGGTCCCCGTCTCCTCAGC48900.16582999949471022No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT48190.16342224285582999No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG47840.162235320569058No Hit
CAGTAATACACTGCCGTGTCTGCGGCGGTCACAGAACTCAGCTGCAGGGA46910.15908149849277825No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC46510.1577250158793246No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCGATGGGACTTACTACTGGG42250.14327847604604307No Hit
CACTATCACCGGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGCCAACAGTATAAAAGTTACCCTCTCACTTT41200.13971770918572723No Hit
CTTTTATACTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCCGGTGATAGTGAGAGTGAACTCTGTC41190.1396837971203909No Hit
GTATAAAAGTTACCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT40280.1365977991747838No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG38000.12886584827809794No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG36640.12425380739235549No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC36160.12262602825621108No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA35830.12150693010011182No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35000.11869222867719546No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34460.11686097714903301No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA33650.11411409985678934No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC33520.11367324300741691No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT32740.11102810191118226No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32140.10899337799100178No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCTCGCTCAGCTCCTGGGGCTC31970.10841687288028397No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31920.10824731255360226No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG31540.10695865407082128No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31160.1056699955880403No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29540.10017624100355296No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTCG19250.042.885970
TACGGAT24350.041.92663670
GTATCAA42500.039.1904141
GTGGTAT7100.034.5558621
TATCAAC48900.034.182232
TCAACGC50700.032.8324284
ATCAACG50850.032.7355773
CAACGCA52200.031.8228075
AACGCAG52350.031.7316256
CTACGGA24450.028.98734369
AGAGTAC58550.028.19644511
CGCAGAG59000.028.1565258
CAGAGTA60450.027.3673410
ACGCAGA61400.027.1103557
GCAGAGT61650.027.0022499
CTGATAC19700.025.12077739
GCTAACC20050.025.10638849
GATACCA20750.024.53256841
GGCTAAC20650.024.36735248
TGATACC20700.024.25064540
Produced by FastQC (version 0.11.5)