FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757148

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757148
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2948803
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTATACTGCATCCAATTTGAAAAATGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAGTTCA85840.291101168847156No Hit
GTAATACACTGCCGTGTCTGCGGCGGTCACAGAACTCAGCTGCAGGGAGA81910.2777737271699737No Hit
GTGTATTACTGTGCGAGACATTATCAGCGCCATTTTGAATACTGGGGCCA66370.22507437763729893No Hit
GTCTCTGGTGGCTCCATCAGCGATGGGACTTACTACTGGGCCTGGATCCG58270.19760560471486227No Hit
GTATAGATCAGGGACTTAGGGGCTTTTTCTGGTTTCTGCTGATACCAGGC56920.19302747589445615No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT56290.19089101577826664No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT52480.1779705188851205No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT50630.17169678679789732No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT50380.17084898516448876No Hit
CAGTAATACACTGCCGTGTCTGCGGCGGTCACAGAACTCAGCTGCAGGGA47490.16104839828228606No Hit
GTATTACTGTGCGAGACATTATCAGCGCCATTTTGAATACTGGGGCCAGGGAATCCTGGTCCCCGTCTCCTCAGC46530.15779284000999727No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG44510.1509426028120563No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC43260.1467035946450136No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCGATGGGACTTACTACTGGG40710.1380560179842465No Hit
CTTTTATACTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCCGGTGATAGTGAGAGTGAACTCTGTC39290.133240504706486No Hit
GTATAAAAGTTACCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT37960.12873020001675256No Hit
CACTATCACCGGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGCCAACAGTATAAAAGTTACCCTCTCACTTT37230.12625461924719963No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA34170.11587752725427912No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC33930.11506363768620691No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33550.11377497920342593No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33490.11357150681140789No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32570.11045159680046447No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA32450.11004465201642835No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG32090.10882381766432006No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30560.10363527166785981No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC30550.10360135960252347No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT30440.1032283268838237No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG29920.10146489948633394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAT23300.049.64869770
TGACTCG18350.041.55819770
GTATCAA46550.037.8578151
GTGGTAT8850.037.4095341
TATCAAC52750.033.136552
CTACGGA24300.033.1073369
ATCAACG53750.032.4559143
TCAACGC53650.032.452154
CAACGCA55650.031.4097565
AACGCAG56000.031.3402966
CGCAGAG61900.028.255588
AGAGTAC61450.028.23842411
CAGAGTA62550.027.9072810
ACGCAGA65050.026.9348747
GCAGAGT65800.026.5288839
TCTATAC30550.026.52033
GATCTAT30850.025.8232651
ATCTATA30850.025.703632
AAGGTTC31400.025.23045340
CAATTTG31000.025.14676516
Produced by FastQC (version 0.11.5)