FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379997
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT11880.31263404711089826No Hit
GACCTAGGACGGTGACCTGGGTCCCAGTTCCGAAGACCCAAGTGGTAATTCCTGCAATTGAGCAGCAGTAATAAT11580.3047392479414311No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA10720.28210749032229204No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC10460.27526533104208717No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC9560.2515809335336858No Hit
ACCTAGGACGGTGACCTGGGTCCCAGTTCCGAAGACCCAAGTGGTAATTC9020.23737029502864496No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC8760.2305281357484401No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG8090.21289641760329686No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG7980.2100016579078256No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTT7930.20868585804624773No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC7820.20579109835077644No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTCTCAGGGAGG7690.20237001871067406No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTGGGTCCCAGTTCCGAAGACCCAAGTGGTAATTCCTGCAATTGAGCAG7360.1936857396242602No Hit
GATTATTACTGCTGCTCAATTGCAGGAATTACCACTTGGGTCTTCGGAACTGGGACCCAGGTCACCGTCCTAGGT7340.19315941967962905No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG6890.18131722092542837No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGTATTATAATCTTGTCTCCTGGTACCAACAACACCCAGGCAAAGT6410.16868554225428095No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC6320.1663171025034408No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG6030.15868546330628927No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG5980.1573696634447114No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGTATTATAATCTTGTCTCCTGGTACCAACAACA5930.15605386358313356No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT5910.1555275436385024No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC5870.15447490374924014No Hit
GTCCCCAAACTCATCATTTATGAGGGCAGTAAGCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGT5590.14710642452440414No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTGGGTC5580.14684326455208857No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT5510.14500114474587958No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT5000.13157998615778546No Hit
GTTTGGGGACTTTGCCTGGGTGTTGTTGGTACCAGGAGACAAGATTATAA4990.1313168261854699No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC4770.1255273067945273No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC4760.12526414682221176No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG4600.12105358726516263No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT4510.11868514751432248No Hit
CTCTCGGGACGTCGCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGC4340.11421142798495779No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGA4300.11315878809569549No Hit
CCCTGAGAATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGC4280.11263246815106434No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC4190.1102640284002242No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT4070.10710610873243735No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC3980.10473766898159721No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACT3930.10342186912001936No Hit
CAAGAGGCAGCGCTCTCGGGACGTCGCCACCATGGCCTGGGCTCTGCTGC3860.10157974931381036No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9550.042.4592171
TATCAAC10450.038.1196752
GGTATCA3900.037.8876081
TCAACGC11250.035.4089434
ATCAACG11400.034.9430353
CAACGCA11800.034.6315965
TCGTATG501.8433946E-434.54971740
AACGCAG12000.034.0543986
ACGCAGA12800.031.6577137
CGCAGAG12850.031.5386838
AGAGTAC12950.031.02992811
CAGAGTA13100.030.67462510
TTATGAC450.004724677730.5249523
ACGGGAC902.0716652E-730.52495216
AGTACGG10300.030.01027513
GAGTACG10400.029.39147212
CGTATGC605.353329E-428.80668641
TACGGGA3000.028.61714215
GCAGAGT14350.028.4812979
TGGTATC1353.2196112E-1027.9812072
Produced by FastQC (version 0.11.5)