FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379997
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTAGGACGGTGACCTGGGTCCCAGTTCCGAAGACCCAAGTGGTAATTCCTGCAATTGAGCAGCAGTAATAAT10630.2797390505714519No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT10290.27079161151272246No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC9580.2521072534783169No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC9370.24658089405968994No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA8920.23473869530548927No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC8410.22131753671739512No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTT7980.2100016579078256No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG7950.20921217799087888No Hit
GATTATTACTGCTGCTCAATTGCAGGAATTACCACTTGGGTCTTCGGAACTGGGACCCAGGTCACCGTCCTAGGT7810.2055279383784609No Hit
ACCTAGGACGGTGACCTGGGTCCCAGTTCCGAAGACCCAAGTGGTAATTC7560.19894893907057162No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC7400.1947383795135225No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7390.1944752195412069No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC7070.18605410042710863No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG6850.18026458103616608No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTGGGTCCCAGTTCCGAAGACCCAAGTGGTAATTCCTGCAATTGAGCAG6700.17631718145143252No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGTATTATAATCTTGTCTCCTGGTACCAACAACACCCAGGCAAAGT6650.17500138158985465No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG6610.17394874170059238No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT6230.16394866275260067No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG6200.16315918283565395No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTCTCAGGGAGG6010.15815914336165812No Hit
GTCCCCAAACTCATCATTTATGAGGGCAGTAAGCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGT5860.15421174377692456No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG5850.153948583804609No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC5810.1528959439153467No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGTATTATAATCTTGTCTCCTGGTACCAACAACA5670.1492117043029287No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC5170.13605370568715017No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC5150.13552738574251902No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTGGGTC4890.12868522646231417No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC4710.1239483469606339No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT4690.12342202701600277No Hit
GTTTGGGGACTTTGCCTGGGTGTTGTTGGTACCAGGAGACAAGATTATAA4650.12236938712674046No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC4430.11657986773579791No Hit
CTCTCGGGACGTCGCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGC4420.11631670776348234No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGA4370.11500090790190448No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT4300.11315878809569549No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG4140.10894822853864634No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT4130.1086850685663308No Hit
CCCTGAGAATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGC4070.10710610873243735No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA4050.10657978878780622No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG3940.10368502909233494No Hit
CTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC3900.10263238920307266No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACT3830.10079026939686367No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGT350.004924552557.26281470
CCCGTTA200.00751790551.464514
GGTATCA4100.040.9772531
ACCCTAT350.001383801939.211058
TATATGC350.001390086139.174932
GTATCAA9850.038.290151
TTCGGGG750.00221212535.6301970
GTGGTAT1550.035.3931241
ATGCTCG400.00257210734.56003640
TATCAAC11050.034.433172
TGGTATC1650.033.2393342
CAACGCA11550.032.946895
TCAACGC11550.032.9425544
ATCAACG11600.032.800563
AACGCAG11700.032.5373426
CTATGTA450.003856591931.8176366
ACGCAGA12450.030.5893427
CGCAGAG12950.029.6732278
CAGAGTA13050.029.44584510
AGAGTAC12900.029.25630811
Produced by FastQC (version 0.11.5)