FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1067804
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGTTTCAGGGAGA47170.44174773647598253No Hit
ACCTACTACAGCCCGTCCCTCAAGAGTCGAGTCTCCATATCCGTAGACACGTCCAAGAACCAGTTCTCCCTGAAA33270.3115740341860491No Hit
GTCTCTGGTGGCTCCATCACCAGTGATGATTACTACTGGGGCTGGATCCG31200.29218845406085764No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT29670.2778599817944117No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT28890.2705552704428903No Hit
GTGTATTACTGTGCGAGACGCCGATATAACTGGAACGAGGGGTGGTTCGA27370.2563204483219767No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT27330.25594584773984735No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC26500.2481728856606643No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG25890.24246022678319243No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCACCAGTGATGATTACTACTGGG23990.22466669913205045No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGTTTCAGGGA22710.2126794805039127No Hit
CTCTAAACTAGACGCCTTATAGATCAGGACCTTGGGGGCTTTCCCTGGCT22520.21090012773879852No Hit
AGTTTAGAGAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGCGACAGAATTCACTCTCACCATCAGCAGC22160.20752872249963478No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG21780.20397001696940636No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21750.20368906653280938No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC21690.20312716565961544No Hit
GTAGTAGGTACTCCCACTATAATAGATACTCCCAATCCACTCCAACCCCTTCCCTGGGGGCTGGCGGATCCAGCC19870.18608283917273205No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18830.17634322403737016No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA18740.1755003727275792No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18360.17194166719735082No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC18330.17166071676075384No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA18280.1711924660330922No Hit
GTATTACTGTGCGAGACGCCGATATAACTGGAACGAGGGGTGGTTCGACCCCTGGGGCCAGGGAGCCCTGGTCAC17320.1622020520619889No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC17280.1618274514798596No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC17220.16126555060666564No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16730.15667669347558166No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16670.1561147926023877No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT16650.15592749231132305No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC16370.15330528823641792No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT15730.14731167892234903No Hit
CCTGTGGGTCTTCCTCCTGCTGGTGGCGGCTCCCAGATGGGTCCTGTCCC15510.14525137572063787No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15090.14131806960828017No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA14760.1382276148057134No Hit
CTCCATATCCGTAGACACGTCCAAGAACCAGTTCTCCCTGAAACTGAGCT14380.134668909275485No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14180.13279590636483848No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG14160.13260860607377384No Hit
ACCAGAGACAGTGCAGGTGAGGGACAGGGTCTCCGAAGGCTTCACTAGTCCTGGGCCCGACTCGTGCAGCTGCAG13810.1293308509801424No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13730.12858164981588383No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG13540.12680229705076962No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT13270.1242737431213968No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA13180.12343089181160588No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT13080.12249439035628262No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12940.12118328831883005No Hit
GATCAGGACCTTGGGGGCTTTCCCTGGCTTCTGCTGATACCAGGCCAACC12910.12090233788223308No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG12890.1207150375911684No Hit
GTCCTGATCTATAAGGCGTCTAGTTTAGAGAGTGGGGTCCCATCAAGGTT12830.12015313671797445No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA12820.12005948657244213No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC12630.11828013380732792No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT12580.11781188307966632No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG12340.11556427958689047No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT12330.11547062944135815No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12300.11518967900476117No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA12190.11415952740390559No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG12090.11322302594858231No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12020.11256747492985604No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT11900.11144367318346812No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC11890.11135002303793581No Hit
GGACTAGTGAAGCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCACCAGTGATGATTA11840.11088177231027417No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11680.10938336998175696No Hit
GTGTATTACTGTGCGAGACGCCGATATAACTGGAACGAGGGGTGGTTCGACCCCTGGGGCCAGGGAGCCCTGGT11640.10900876939962764No Hit
ACTACAGCCCGTCCCTCAAGAGTCGAGTCTCCATATCCGTAGACACGTCC11590.10854051867196601No Hit
CGGTGGGCATGTGTGAGTTTTGTCACAAGATTTGGGCTCAACTTTCTTGT11540.10807226794430438No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11380.10657386561578716No Hit
GTCTGCATCTGTAGGAGACAGACTCACCATCACTTGCCGGGCCAGTCAGA11310.10591831459706089No Hit
GAGGAAGACTGACGGTCCCCCCAGGAGTTCAGGTGCTGGGCACGGTGGGC11200.1048881629962053No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG11050.1034834108132204No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT10950.10254690935789713No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10930.10235960906683249No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG10920.10226595892130017No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10900.1020786586302355No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG10800.10114215717491225No Hit
GTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCACCAGTGATGATTACT10780.1009548568838476No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)