FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1067804
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGTTTCAGGGAGA45040.42180025547759703No Hit
ACCTACTACAGCCCGTCCCTCAAGAGTCGAGTCTCCATATCCGTAGACACGTCCAAGAACCAGTTCTCCCTGAAA31530.29527890886342434No Hit
GTCTCTGGTGGCTCCATCACCAGTGATGATTACTACTGGGGCTGGATCCG31280.2929376552251162No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT31150.291720203333196No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT30080.2816996377612371No Hit
GTGTATTACTGTGCGAGACGCCGATATAACTGGAACGAGGGGTGGTTCGA26040.24386497896617731No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC25650.24021262329041662No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT24500.22944285655419908No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG22970.2151143842877532No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGTTTCAGGGA21970.20574936973452054No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCACCAGTGATGATTACTACTGGG21830.204438267697068No Hit
CTCTAAACTAGACGCCTTATAGATCAGGACCTTGGGGGCTTTCCCTGGCT20850.19526055343490004No Hit
GTAGTAGGTACTCCCACTATAATAGATACTCCCAATCCACTCCAACCCCTTCCCTGGGGGCTGGCGGATCCAGCC20570.1926383493599949No Hit
AGTTTAGAGAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGCGACAGAATTCACTCTCACCATCAGCAGC20380.1908589965948807No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG20070.1879558420833786No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19580.18336698495229461No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18820.17624957389183782No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18380.1721289674884155No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC17970.1682893115215901No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC16860.1578941453675019No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16840.15770684507643726No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT16370.15330528823641792No Hit
GTATTACTGTGCGAGACGCCGATATAACTGGAACGAGGGGTGGTTCGACCCCTGGGGCCAGGGAGCCCTGGTCAC16320.15283703750875627No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC15980.1496529325606572No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT15700.14703072848575208No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT15430.14450217455637926No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15180.14216092091807112No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA14960.14010061771635993No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14900.139538716843166No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT14400.13485620956654965No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT14260.1335451075290971No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14250.1334514573835648No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT14140.1324213057827092No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14040.13148480432738593No Hit
CTCCATATCCGTAGACACGTCCAAGAACCAGTTCTCCCTGAAACTGAGCT13800.1292372008346101No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13710.12839434952481918No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13680.12811339908822217No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT13550.12689594719630196No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13540.12680229705076962No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13450.1259594457409787No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG13100.12268169064734726No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA13080.12249439035628262No Hit
CCTGTGGGTCTTCCTCCTGCTGGTGGCGGCTCCCAGATGGGTCCTGTCCC13070.1224007402107503No Hit
GTCCTGATCTATAAGGCGTCTAGTTTAGAGAGTGGGGTCCCATCAAGGTT12970.12146423875542703No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG12820.12005948657244213No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12770.1195912358447805No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG12710.11902933497158655No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA12610.11809283351626328No Hit
GTGTATTACTGTGCGAGACGCCGATATAACTGGAACGAGGGGTGGTTCGACCCCTGGGGCCAGGGAGCCCTGGT12470.11678173147881073No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA12310.1152833291502935No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA12300.11518967900476117No Hit
GATCAGGACCTTGGGGGCTTTCCCTGGCTTCTGCTGATACCAGGCCAACC12230.11453412798603489No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA12140.11369127667624394No Hit
ACCAGAGACAGTGCAGGTGAGGGACAGGGTCTCCGAAGGCTTCACTAGTCCTGGGCCCGACTCGTGCAGCTGCAG12120.1135039763851793No Hit
ACTACAGCCCGTCCCTCAAGAGTCGAGTCTCCATATCCGTAGACACGTCC12090.11322302594858231No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG11840.11088177231027417No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11790.11041352158261254No Hit
GTGGGCATGTGTGAGTTTTGTCACAAGATTTGGGCTCAACTTTCTTGTCC11770.11022622129154788No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11730.10985162070941859No Hit
GGACTAGTGAAGCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCACCAGTGATGATTA11500.10769766736217506No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT11460.10732306678004579No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA11440.10713576648898111No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC11310.10591831459706089No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC11260.10545006386939926No Hit
CTATTATAGTGGGAGTACCTACTACAGCCCGTCCCTCAAGAGTCGAGTCTCCATATCCGTAGACACGTCCAAGAA10930.10235960906683249No Hit
GTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCACCAGTGATGATTACT10830.10142310761150923No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10750.1006739064472506No Hit
TCCGTAGACACGTCCAAGAACCAGTTCTCCCTGAAACTGAGCTCTGTGACCGCCGCAGACACGGCTGTGTATTA10690.10011200557405665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2900.041.5882231
TACGGAT14000.039.1789170
GTATCAA16000.037.904691
TATCAAC18400.032.7687232
ATCAACG18900.031.5372333
ATTAATC450.00451485930.81477247
AACGCAG19600.030.5881216
TCAACGC19550.030.488684
CAACGCA19850.030.2014685
CGCAGAG20650.028.8808148
CGATATA13300.028.77772522
TGTTAGA5850.028.58582170
AGAGTAC21000.028.56362511
CAGAGTA21200.028.29415910
GCCGATA13650.028.29244620
TTGGAGT16600.028.2069770
CGCCGAT13700.028.18390719
TAAGGAT950.007003905728.16455370
GATATAA13600.028.14292123
ATATAAC13900.027.53681224
Produced by FastQC (version 0.11.5)