FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences921667
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTATACTGCATCCAATTTGAAAAATGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAGTTCA41740.4528750622513338No Hit
GTAATACACTGCCGTGTCTGCGGCGGTCACAGAACTCAGCTGCAGGGAGA32340.35088594904667303No Hit
GTGTATTACTGTGCGAGACATTATCAGCGCCATTTTGAATACTGGGGCCA29070.3154067575382432No Hit
GTCTCTGGTGGCTCCATCAGCGATGGGACTTACTACTGGGCCTGGATCCG27200.29511743395391177No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25620.27797458301100075No Hit
GTATAAAAGTTACCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT23640.2564917698040616No Hit
CTTTTATACTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCCGGTGATAGTGAGAGTGAACTCTGTC22970.24922233301181446No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT22410.24314638584217507No Hit
GTATTACTGTGCGAGACATTATCAGCGCCATTTTGAATACTGGGGCCAGGGAATCCTGGTCCCCGTCTCCTCAGC22300.24195289621956736No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT22270.24162739904976527No Hit
GTATAGATCAGGGACTTAGGGGCTTTTTCTGGTTTCTGCTGATACCAGGC21820.23674494150273365No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC21390.23207948206890341No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT20970.2275225216916739No Hit
CACTATCACCGGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGCCAACAGTATAAAAGTTACCCTCTCACTTT20410.2214465745220345No Hit
CAGTAATACACTGCCGTGTCTGCGGCGGTCACAGAACTCAGCTGCAGGGA20390.22122957640883312No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18700.20289323584331437No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17750.19258582546624756No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG17500.18987334905123No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG17350.18824586320221945No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCGATGGGACTTACTACTGGG16490.178914944334559No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCTCGCTCAGCTCCTGGGGCTC15630.16958402546689857No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15280.16578655848587398No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15050.1632910801840578No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14910.16177209339164794No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG14850.16112109905204375No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14360.15580464527860932No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT14230.15439415754280017No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13820.14994569622217135No Hit
GGATATTAGCAGCTGGTTAGCCTGGTATCAGCAGAAACCAGAAAAAGCCCCTAAGTCCCTGATCTATACTGCATC13770.14940320093916784No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG13620.14777571509015727No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13550.14701622169395237No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT13190.14311025565632707No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA13180.14300175659972636No Hit
GCATGGACATGAGGGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT13100.14213376414692072No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13050.14159126886391724No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12950.1405062782979102No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC12920.14018078112810808No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC12880.13974678490170528No Hit
CAGCATGGACATGAGGGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACGT12520.13584081886407998No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12280.1332368415056631No Hit
GAGTTCACTCTCACTATCACCGGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGCCAACAGTATAAAAGTTAC12270.1331283424490624No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG12200.1323688490528575No Hit
CTATAACCCGTCCCTCAAGAGTCGGGTCACCATATCCGTAGACACGTCCA12060.13084986226044765No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA11620.1260759037700167No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11560.1254249094304125No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC11510.124882414147409No Hit
GTCTATTATAGTGGGAGCACTTACTATAACCCGTCCCTCAAGAGTCGGGT11460.12433991886440547No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA11340.12303793018519704No Hit
GTCTCCATCCTCACTGTCTGCATCTGTAGGAGACACAGTCACCATCACTTGTCGGGCGAGTCAGGATATTAGCAG11220.12173594150598861No Hit
GTCTGCATCTGTAGGAGACACAGTCACCATCACTTGTCGGGCGAGTCAGG11220.12173594150598861No Hit
ACTATAACCCGTCCCTCAAGAGTCGGGTCACCATATCCGTAGACACGTCC11040.11978295848717596No Hit
GTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTTCTTCCTCCTGCTGGTGGCGGCTCCCAGATGGGTC10990.11924046320417245No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT10990.11924046320417245No Hit
GTGATAGTGAGAGTGAACTCTGTCCCAGATCCACTGCCGCTGAACCTTGA10750.11663648584575557No Hit
GTATCAACGCAGAGTACGGGATGCTTTCTGAGAGTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTT10660.11565999433634924No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10610.11511749905334574No Hit
ATACTGCATCCAATTTGAAAAATGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAGTTCACTCTCA10590.11490050094014433No Hit
CTATTATAGTGGGAGCACTTACTATAACCCGTCCCTCAAGAGTCGGGTCACCATATCCGTAGACACGTCCAAGAA10550.11446650471374151No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA10480.1137070113175366No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGA10380.11262202075152955No Hit
GGACACAGCATGGACATGAGGGTCCTCGCTCAGCTCCTGGGGCTCCTGCT10180.11045203961951551No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC10170.11034354056291482No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA10050.10904155188370637No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9980.10828205848750144No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCTCGCTCAGCTCCTGG9960.10806506037430005No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG9920.10763106414789723No Hit
GTCAGGACACAGCATGGACATGAGGGTCCTCGCTCAGCTCCTGGGGCTCC9830.10665457263849092No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC9700.10524408490268178No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTC9700.10524408490268178No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9540.10350809999707053No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG9530.10339960094046984No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG9530.10339960094046984No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA9440.1024231094310635No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT9330.10122961980845577No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT9330.10122961980845577No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9280.10068712452545225No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATT200.007293739451.8659538
GTGGTAT2050.048.8425181
TGACTCG6650.048.81322570
TCGTATG451.8270475E-646.1231440
GTATCAA17400.042.06691
TACGGAT9750.041.94933370
ATGTGTA1550.040.0606111
GTGTATA1500.039.09618813
ATAGGCA851.023175E-438.18916770
CGTATGC557.253004E-637.74121541
TATCAAC20500.035.8428462
ATCAACG21750.033.7829133
CAACGCA21850.033.62835
AACGCAG21750.033.6243066
TCAACGC22000.033.2422144
TGTATAA1800.032.58015414
ATACGAG652.1840753E-532.1306856
CGTCAGA1850.031.6996145
AGAGTAC23450.031.18672411
CAGAGTA23500.031.1203710
Produced by FastQC (version 0.11.5)