FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences921667
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTATACTGCATCCAATTTGAAAAATGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAGTTCA37950.41175391979966736No Hit
GTAATACACTGCCGTGTCTGCGGCGGTCACAGAACTCAGCTGCAGGGAGA34210.37117527263100447No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26580.2883904924446682No Hit
GTGTATTACTGTGCGAGACATTATCAGCGCCATTTTGAATACTGGGGCCA25830.28025306319961546No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT25690.2787340764072056No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT23280.25258580376643625No Hit
GTCTCTGGTGGCTCCATCAGCGATGGGACTTACTACTGGGCCTGGATCCG23090.2505243216910229No Hit
CTTTTATACTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCCGGTGATAGTGAGAGTGAACTCTGTC23060.2501988245212208No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT21460.23283897546510832No Hit
GTATAAAAGTTACCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT20330.2205785820692289No Hit
CAGTAATACACTGCCGTGTCTGCGGCGGTCACAGAACTCAGCTGCAGGGA20040.21743210942780852No Hit
GTATTACTGTGCGAGACATTATCAGCGCCATTTTGAATACTGGGGCCAGGGAATCCTGGTCCCCGTCTCCTCAGC19700.21374314150338464No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC19310.20951167829595724No Hit
GTATAGATCAGGGACTTAGGGGCTTTTTCTGGTTTCTGCTGATACCAGGC19220.20853518678655092No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG18730.20321873301311646No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC17880.1939963132020567No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16710.18130192357977448No Hit
CACTATCACCGGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGCCAACAGTATAAAAGTTACCCTCTCACTTT16330.17717895942894776No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15730.17066901603290557No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG15530.16849903490089155No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15080.1636165773538599No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14750.16003610848603672No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG14520.15754063018422054No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCGATGGGACTTACTACTGGG14390.15613014244841142No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT14250.15461115565600156No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT13810.14983719716557065No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCTCGCTCAGCTCCTGGGGCTC13780.14951169999576852No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13710.14875220659956362No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA13480.14625672829774744No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13270.14397824810913268No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC13270.14397824810913268No Hit
GTGATAGTGAGAGTGAACTCTGTCCCAGATCCACTGCCGCTGAACCTTGA13250.1437612499959313No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG12770.13855329527909754No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12610.1368173103734863No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA12190.13226034999625677No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12180.1321518509396561No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA12060.13084986226044765No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT12040.13063286414724623No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11950.1296563726378399No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT11790.12792038773222866No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT11570.12553340848701322No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA11470.12444841792100618No Hit
GGATATTAGCAGCTGGTTAGCCTGGTATCAGCAGAAACCAGAAAAAGCCCCTAAGTCCCTGATCTATACTGCATC11460.12433991886440547No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT11460.12433991886440547No Hit
GAGTTCACTCTCACTATCACCGGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGCCAACAGTATAAAAGTTAC11450.12423141980780476No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT11420.12390592263800265No Hit
CTATAACCCGTCCCTCAAGAGTCGGGTCACCATATCCGTAGACACGTCCA11350.12314642924179775No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA11100.12043395282678017No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC11040.11978295848717596No Hit
GTATCAACGCAGAGTACGGGATGCTTTCTGAGAGTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTT10790.11707048207215838No Hit
GCATGGACATGAGGGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT10700.11609399056275206No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10670.11576849339294995No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10490.1138155103741373No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA10330.11207952546852605No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10270.11142853112892184No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10270.11142853112892184No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10250.11121153301572043No Hit
CAGCATGGACATGAGGGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACGT10190.1105605386761162No Hit
GTCTGCATCTGTAGGAGACACAGTCACCATCACTTGTCGGGCGAGTCAGG10090.10947554811010919No Hit
GTCTCCATCCTCACTGTCTGCATCTGTAGGAGACACAGTCACCATCACTTGTCGGGCGAGTCAGGATATTAGCAG10040.10893305282710568No Hit
GTCTATTATAGTGGGAGCACTTACTATAACCCGTCCCTCAAGAGTCGGGT10040.10893305282710568No Hit
GTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTTCTTCCTCCTGCTGGTGGCGGCTCCCAGATGGGTC9980.10828205848750144No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG9770.10600357829888668No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG9710.10535258395928247No Hit
GGACACAGCATGGACATGAGGGTCCTCGCTCAGCTCCTGGGGCTCCTGCT9660.10481008867627897No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC9630.10448459150647685No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGA9620.10437609244987615No Hit
CTATTATAGTGGGAGCACTTACTATAACCCGTCCCTCAAGAGTCGGGTCACCATATCCGTAGACACGTCCAAGAA9510.1031826028272684No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)