FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1234703
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG1050.042.85089540
GTATCAA29400.039.9919931
GGTATCA12450.037.3878441
GTGGTAT6000.033.9052351
TATCAAC35100.033.6708372
CGTATGC1450.033.43319741
TCAACGC35700.032.9119074
CAACGCA35750.032.865885
ATCAACG36100.032.642683
TGGTATC6100.031.631892
AACGCAG37150.031.6273276
CTCGTAT1251.2187229E-1030.44099839
ACGCAGA39700.029.595857
AGAGTAC41000.028.65860411
CAGAGTA41150.028.47040210
CGCAGAG41550.028.2781058
TATACAC2150.027.244413
GCAGAGT44400.026.6181629
TCTCGTA1501.0168151E-925.354138
AGTACGG37300.024.20343213
Produced by FastQC (version 0.11.5)