FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1234703
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33450.043.4074131
GGTATCA12700.038.1999631
TCAACGC38650.037.2912144
TATCAAC38900.036.9631272
ATCAACG39050.036.909233
CAACGCA39450.036.6221925
AACGCAG41150.035.3614586
ACGCAGA43350.033.495667
AGAGTAC43350.033.34636711
CAGAGTA44750.032.4569510
CGCAGAG45400.031.9909678
TTAACGG2500.031.75482235
TGGTATC5400.031.2136342
ATTAACG450.004396340330.9831452
GTGGTAT5850.030.5840551
GCAGAGT47650.030.4816069
TAACGGC2650.029.9768736
GAGTACG39050.028.73325712
AGTACGG38700.028.72631513
TTTAACG2850.027.84945134
Produced by FastQC (version 0.11.5)