FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences292561
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC1350.038.2404752
GGTATCA3300.037.551611
GTATCAA7250.037.5084461
GTGGTAT1400.036.8810461
AATGGAC501.8847076E-434.4164283
ACGTGAT951.9150422E-434.31028770
ATCAACG8150.033.3607063
CGTGGAT800.003420700632.59477270
TCAACGC8350.032.561654
CAACGCA8550.032.2025035
AACGCAG8650.031.8302176
ACGATTA553.0233117E-431.75439656
GTAGTTT1053.4457908E-431.04263970
TATCAAC8850.030.7220062
CAGAGTA9100.030.26654410
AGAGTAC9150.030.10115211
ACGCAGA9350.029.4522427
CGATTAA605.0237105E-429.11829857
CGCAGAG9650.028.8933358
TATACTG605.489812E-428.6803535
Produced by FastQC (version 0.11.5)