FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences826428
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGCGAGAGTGAATTCTGTC24410.2953675335298417No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19700.2383752728610357No Hit
GTATAATACTTATTCTCCGTGGACATTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCAT19220.23256714438523382No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT14650.17726892118853668No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14590.17654290512906146No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC13750.16637868029640815No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13570.16420063211798247No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12470.1508903376942698No Hit
GTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTGCTACAGA11420.13818505665345318No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11240.13600700847502747No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10670.12910985591001273No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10390.12572178096579495No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10240.12390674081710687No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10200.12342273011079004No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG10190.12330172743421083No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10190.12330172743421083No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT10150.12281771672789403No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9830.11894563107735943No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT9770.1182196150178842No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9730.11773560431156736No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9680.11713059092867135No Hit
GAGTATTACTAACTATTTGGCCTGGTATCAACAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGACGT9570.11579956148630008No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC9570.11579956148630008No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9490.11483154007366643No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9440.1142265266907704No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG9090.1099914330104982No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8410.10176325100311218No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAT305.483771E-447.3290356
ATACGAA850.003360966532.72488470
CGATACG450.00446910630.87810745
ATCAACG27300.030.5617433
TCAACGC27700.030.1204224
CAACGCA28000.029.9188295
AACGCAG28350.029.674486
GTATCAA30250.028.9302751
AGAGTAC29550.028.6101911
ACGCAGA29700.028.3427937
CGCAGAG29900.028.2752888
GTATATA500.00840034927.1361481
GCAGAGT31400.026.816439
TATCAAC33300.026.1754722
TTATACT10200.025.9358274
GTGGTAT7150.025.6180421
TATACTG10450.025.3153535
AGTACGG29950.024.94047713
AATCATC9900.024.8043132
CAGTAAT10050.024.6579416
Produced by FastQC (version 0.11.5)