FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences831072
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT17240.2074429170998421No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG15260.18361826652805052No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15120.1819336952754996No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13920.1674945131107774No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12590.15149108621154364No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC11790.14186496476839552No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11720.14102267914212005No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG11710.1409023526240807No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11600.13957876092564783No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC11100.13356243502368026No Hit
GTCATGGACCTCCTGTGCAAGAACATGAAGCACCTGTGGTTTTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC10860.13067459859073582No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10770.12959165992838168No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10760.12947133341034228No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10690.12862904778406684No Hit
GTCTCCATCCTCCCTGTCTGCTTCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTTATGACATTAGCAA10310.1240566400985715No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10300.12393631358053213No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10060.12104847714758768No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCCCGCCGAAAGTG9700.11671672249817103No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA9620.11575411035385623No Hit
CAATAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGAATTGGTTTTTGGACGTGTCTA9540.11479149820954142No Hit
GTATGATCATCTCCCTCCGCTCACTTTCGGCGGGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT9510.11443051865542336No Hit
ATAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA9430.11346790651110854No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9130.109858110969928No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG9120.10973778445188866No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9030.10865484578953448No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT9020.10853451927149513No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8840.10636864194678682No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG8370.10071329559893727No Hit
GTGCAAGAACATGAAGCACCTGTGGTTTTTCCTCCTGCTGGTGGCAGCTC8320.10011166300874053No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGCT200.00717027552.089950
GTGGTAT2100.044.296481
GTATCAA15950.039.312931
GTACATA501.8760013E-434.4528161
TATCAAC18900.032.984572
ATCAACG19150.032.3741073
AACGCAG19450.032.0576326
CAACGCA19500.031.9696625
GTCGTAA652.3319835E-531.82366820
TCAACGC19550.031.7117214
CGCAGAG20750.030.0654838
AGAGTAC20800.029.99501811
CAGAGTA21100.029.56854810
ACGCAGA21850.028.5467347
GCAGAGT21900.028.4884199
CAACGCG500.007662817427.6521237
CCCTTAT500.007785522427.5622541
CGTAAAC658.684722E-426.52291722
AGTACGG20350.026.42372713
GAGTACG20800.026.01777612
Produced by FastQC (version 0.11.5)