FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences651685
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32310.49579167849497835No Hit
GTATAACAGTGCCCCCCTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT17800.2731380958591958No Hit
CTGTTATACTGTTGACAGGAATAAGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC15610.2395329031664071No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT10480.160813890146313No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9750.1496121592487168No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC8880.13626215119267746No Hit
GTTATACTGTTGACAGGAATAAGTTGCAACATCTTCAGGCTGCAGGCTGC8650.13273283871809233No Hit
CAGTATAACAGTGCCCCCCTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT8520.13073800992810944No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT8330.12782249092736522No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8310.12751559419044478No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8260.12674835234814366No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8090.12413973008431989No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC8040.12337248824201878No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7040.10802765139599653No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT6890.1057259258690932No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT6600.10127592318374674No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGCG200.00801947850.6345730
GTGGTAT4050.038.9877131
GTATCAA23000.037.5396461
TACGGAT1803.930836E-935.47674670
CCTATAC601.609191E-533.59583
TATCAAC26600.032.4590952
TGTGATT2001.0992153E-831.92907170
TCAACGC27500.031.39684
AACGCAG27750.031.2350066
ATCAACG27850.031.0022223
CAACGCA27900.030.9466675
CCCTATA553.8039067E-430.5416322
CTACGGA1651.5097612E-1029.8743969
CAGAGTA28850.029.61008310
AGAGTAC28950.029.50780111
CGCAGAG29550.029.4709368
GCAGAGT29950.028.8594479
GGTATCA13150.028.6139091
ACGCAGA31600.027.4463257
TGTTATA5600.026.3966982
Produced by FastQC (version 0.11.5)