FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1007632
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12750.040.161571
GTATCAA31800.039.986121
TATCAAC34900.035.9170152
CAACGCA35850.035.348425
ATCAACG35600.035.307253
TCAACGC35900.035.203534
GTGGTAT4600.034.3664441
AACGCAG37700.033.6138156
ACGCAGA39950.031.8066397
CGCAGAG40450.031.4165948
AGAGTAC41300.030.43886811
CAGAGTA41750.030.11078510
GCAGAGT43200.029.49629
TGGTATC5600.027.596712
GAGTACG33650.026.84529312
AGTACGG33850.026.6866813
TTAACGG1101.1550201E-625.08471135
CGATTGT1751.42113695E-524.44618470
TAACGGC1151.6928443E-624.0072336
GTACGGG37450.023.75447514
Produced by FastQC (version 0.11.5)