FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1007632
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13390.1328858154564365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31650.041.72681
GGTATCA11750.037.6599661
TATCAAC34950.037.381352
TCAACGC36400.035.798054
CAACGCA36500.035.606035
ATCAACG36700.035.5988583
AACGCAG37800.034.475626
ACGCAGA39900.032.6724667
CGCAGAG40300.032.35628
AGAGTAC39850.032.03588511
CAGAGTA40700.031.61979110
GCAGAGT42300.030.8294189
ACGGGTA2800.028.17999816
GTGGTAT5000.028.1281991
GAGTACG34250.026.95341712
AGTACGG34250.026.85321813
TATACGA700.001335752724.6364436
GAGTACT14500.024.37762312
GTACTTT14400.024.30859414
TGGTATC6000.024.0035342
Produced by FastQC (version 0.11.5)