FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2472770
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATGACTGGCCTCCGGGGACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT93960.3799787283087388No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT81640.33015605980337837No Hit
GTCATTATACTGCTGACAGAAATAAAGTGCAAAATCTTCAGACTGCAGACTGCTGATGGTGAGAGTGAACT76150.307954237555454No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATTCCA72040.2913332012277729No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCAGTGGCCCTGGT61040.24684867577655828No Hit
CTCCTGTAGGGCCAGTCAGAGTGTTAATACCAACTTAGCCTGGTTCCAGCAAAAACCTGGCCAGGCTCCCAGGCT59260.23965027074899806No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGTCTGCAGTCTGAAGATT58150.2351613777261937No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC54910.22205866295692683No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTCCCCGGAGGCCAGTCATTATACTG53920.21805505566631755No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG53660.21700360324656154No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT52670.2129999959559522No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC51740.20923903153144044No Hit
GAGTTCACTCTCACCATCAGCAGTCTGCAGTCTGAAGATTTTGCACTTTATTTCTGTCAGCAGTATAATGACTGG51730.20919859105375752No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC49490.2001399240527829No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC49260.19920979306607567No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT44900.18157774479632152No Hit
GTCTCCAGCCACTCTGTCTGTGTCTCCAGGGGAACGAGCCACCCTCTCCTGTAGGGCCAGTCAGAGTGTTAATAC44340.17931307804607788No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT43100.1742984588133955No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC42200.17065881582193249No Hit
TCCATGGTGCGACCACCAGGGCCACTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT40960.16564419658925011No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT40940.16556331563388427No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT40640.16435010130339658No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCA40580.16410745843729907No Hit
GTGTTAATACCAACTTAGCCTGGTTCCAGCAAAAACCTGGCCAGGCTCCCAGGCTCCTCATCCATGGTGCGACCA39970.16164058929864078No Hit
CCAGTGGCCCTGGTGGTCGCACCATGGATGAGGAGCCTGGGAGCCTGGCC39290.15889063681620208No Hit
GTCTGTGTCTCCAGGGGAACGAGCCACCCTCTCCTGTAGGGCCAGTCAGAGTGTTAATACCAACTTAGCCTGGTT38180.1544017437933977No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC37640.15221795799851986No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG36980.149548886471447No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT35900.1451813148816914No Hit
CACCATGGATGAGGAGCCTGGGAGCCTGGCCAGGTTTTTGCTGGAACCAGGCTAAGTTGGTATTAACACTCTGA34810.14077330281425285No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC34320.13879171940778964No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT34170.13818511224254582No Hit
CTGCTACTCTGGCTCCCAGATTCCACTGGAGAAATACTGATGACGCAGTC32720.13232124297852207No Hit
GGAGAAATACTGATGACGCAGTCTCCAGCCACTCTGTCTGTGTCTCCAGG32090.12977349288449794No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA32050.12961173097376627No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT31410.12702354040205924No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC31180.12609340941535202No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG31080.1256890046385228No Hit
CCTCATCCATGGTGCGACCACCAGGGCCACTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTT29630.11982513537449903No Hit
CCTGTAGGGCCAGTCAGAGTGTTAATACCAACTTAGCCTGGTTCCAGCAAAAACCTGGCCAGGCTCCCAGGCTC29440.1190567662985235No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA28920.11695386145901154No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28750.11626637333840187No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28730.11618549238303602No Hit
ACCCTCTCCTGTAGGGCCAGTCAGAGTGTTAATACCAACTTAGCCTGGTT28480.11517448044096297No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA28390.11481051614181667No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC28130.11375906372206067No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA28040.11339509942291438No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG28000.1132333375121827No Hit
CTCCCAGGCTCCTCATCCATGGTGCGACCACCAGGGCCACTGGTGTCCCA26950.10898708735547584No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26350.10656065869450049No Hit
CTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT26190.10591361105157374No Hit
CTACAGGAGAGGGTGGCTCGTTCCCCTGGAGACACAGACAGAGTGGCTGG25750.10413423003352515No Hit
GCCTGGGAGCCTGGCCAGGTTTTTGCTGGAACCAGGCTAAGTTGGTATTA25420.10279969426998871No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25150.10170780137254982No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG24870.10057546799742798No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA24830.1004137060866963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT7250.045.091781
TGGTATC7900.041.3752
GGTATCA18750.039.8263241
GTATCAA41450.036.446161
ATCAACG49700.030.3220983
TCAACGC49850.030.230864
TATCAAC50350.030.0673182
CAACGCA54550.027.752335
AACGCAG54850.027.6050056
CGCAGAG55650.027.2164218
AGAGTAC56050.026.77816411
GTACCGA658.789814E-426.4708846
GCAGAGT59100.025.6292049
GTAGTTT4600.024.86040170
TTCGGGG10850.024.79975170
CGCTTAT700.001346449524.60448824
ACGCAGA63400.024.0504347
AATGGTA2852.194065E-823.60327170
TGTTAGA19100.023.59709470
AATAGCG3750.023.38600562
Produced by FastQC (version 0.11.5)