FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences642804
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT17570.27333370669753143No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16720.260110391347907No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12560.1953939303426861No Hit
GTATTACTGCCAACAGTATGCTAGCTCACCTCGGACGTTCGGCCAAGGGA12390.1927492672727612No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12000.18668209905352176No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10560.16428024716709913No Hit
GTCTGGGACAGACTTCACTCTCACTATCAGCAGACTGGAGCCTGAGGATT10430.162257857760686No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10380.16148001568129633No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA10050.15634625795732446No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9960.15494614221442307No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9900.15401273171915544No Hit
GTGTATTACTGCCAACAGTATGCTAGCTCACCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAGCGAA9150.14234510052831034No Hit
CTTATATGGTGCATCCAACAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA8830.13736691122021644No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8720.13565565864555915No Hit
CCTCTTATATGGTGCATCCAACAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTT8580.13347770082326804No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGTT8050.12523257478173752No Hit
GTGCAGCCACAGTTCGCTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC7970.12398802745471403No Hit
ATATAAGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGATACCAGGCTAAGTACCTGCTGGTAACACTCTGA7730.1202543854736436No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7520.11698744874020697No Hit
CTCTTATATGGTGCATCCAACAGGGCCACTGGCATCCCAGACAGGTTCAG7520.11698744874020697No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7500.11667631190845111No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7460.11605403824493936No Hit
CAGTATGCTAGCTCACCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAGCGAACTGTGGCTGCACCAT7450.11589846982906142No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7250.11278710151150272No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG7200.11200925943211305No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7000.10889789111455436No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG6680.10391970180646044No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6680.10391970180646044No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTACCAGCAGGTACTTAGCCTGGTATC6680.10391970180646044No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC6620.10298629131119284No Hit
CCATATAAGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGATACCAGGCTAAGTACCTGCTGGTAACACTCTGA6440.10018605982539No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC3350.054.4542243
CTTATAC3400.052.6861841
TTATACA3900.047.657332
GTATCAA10900.044.8780671
TCGTATG4000.042.40096340
TATCTCG3800.040.90911536
ACGAGAC4500.040.5602322
CCCACGA4600.039.66922819
CGAGACG4650.039.2518423
ATGCCGT4550.038.8669544
CGTATGC4300.038.65594541
TGCCGTC4600.038.468645
TATGCCG4550.038.08694543
GACGGAC4850.037.63613526
ACTCCTA4800.037.35718531
TCAACGC13100.037.04664
ATCTCCG4950.036.8528610
GCCGTCT4550.036.63232846
ATCAACG13250.036.6272053
TATCAAC13400.036.2171972
Produced by FastQC (version 0.11.5)