FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences860716
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11050.042.4003451
GTATCAA29650.042.060721
CGAATCG350.001363824539.3328716
TATCAAC33850.036.706032
ATCAACG35350.035.0511253
GTGGTAT3750.034.909621
TCAACGC35750.034.7552224
CAACGCA35800.034.6105425
AACGCAG36800.033.7635656
ACGCAGA38000.032.6048777
AGAGTAC39250.031.6560311
CGCAGAG39350.031.4881178
CAGAGTA39950.031.27366310
TGGTATC3750.031.2058892
GCAGAGT42250.029.4897279
AGTACGG31450.028.12557413
GAGTACG31550.028.03642712
ACGGGTA2350.027.82591216
CGATAGA900.00865682526.96715770
TCGTATG658.450643E-426.64742340
Produced by FastQC (version 0.11.5)