FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757184

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757184
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences860716
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATA200.006829635252.7351260
GTATCAA31350.044.4981881
TATCAAC34700.040.291912
ATCAACG35700.039.067063
GGTATCA11200.038.9618451
TCAACGC35900.038.7537274
CAACGCA36700.037.908965
AACGCAG37850.036.8543476
ACGCAGA39850.034.9286237
GTGGTAT3850.034.8062861
AGAGTAC41500.033.70748511
CGCAGAG41400.033.6228648
CAGAGTA42000.033.30620210
TGGTATC3950.033.0475582
GCAGAGT43600.032.0051279
ACGGGTA1850.029.71852916
AGTACGG33150.029.54890313
GAGTACG33300.029.31258612
TACGGGT7900.026.53580515
GTACGGG37100.026.495514
Produced by FastQC (version 0.11.5)