FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1481720
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35400.2389115352428259No Hit
GAGTTACAGTACCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT30410.20523445725238237No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT28260.1907242933887644No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27970.18876710849553222No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT27190.18350295602408012No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25010.16879032475771402No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24570.16582080285074105No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC24290.16393110709175823No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACCGATTTCA24030.1621763896012742No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23130.15610236751882947No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22540.15212050859811568No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22170.14962341063088844No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21150.14273951893745107No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC21140.14267202980320168No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG21050.1420646275949572No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG20710.13976999703047807No Hit
GTGTATTACTGTGCGAGAGAAAGTTATTACGATTTTTGGAGTGGTTATTA20540.13862268174823852No Hit
GTAATACACGGCCGTGTCCTCGTCTGTCAGGCTGTTCATTTGCAGATATA19440.1311988769808061No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAGAATCTTCAGGTTGCAGACTGCTGATGGTAAGAGTGAAATCGGTC19170.12937667035607267No Hit
GTTACAGTACCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA18960.12795939853683558No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18950.12789190940258618No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC17770.11992819156115865No Hit
GTACTGTAACTCTGTTGACAGTAGTAAGTTGCAGAATCTTCAGGTTGCAG17730.11965823502416112No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17320.11689118051993629No Hit
ACTTACTACTGTCAACAGAGTTACAGTACCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA17130.11560888696919797No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16770.11317927813622006No Hit
GACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATCCAGTTTGCAA16650.11236940852522743No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16320.1101422670949977No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT16230.10953486488675324No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG16230.10953486488675324No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTCTCTGCTGATACCAATT15990.10791512566476796No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15850.10697027778527658No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15690.1058904516372864No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC15560.10501309289204439No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15500.10460815808654807No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT15430.10413573414680237No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT15310.10332586453580972No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC15280.10312339713306158No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA15170.10238101665631834No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG15140.10217854925357017No Hit
ATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACCGATTTCACTCTTA15010.10130119050832816No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAG14960.10096374483708123No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14950.10089625570283184No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14950.10089625570283184No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC14950.10089625570283184No Hit
CTCCTGTACAGCCTCTGGATTTATTTTTAGTGATTATTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG14840.1001538752260886No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTAC459.699589E-538.54353347
GTATCAA19850.036.6930921
CGTCTAA501.7913226E-434.7268950
GTGGTAT3150.032.8755531
TATCAAC22450.032.2690962
TCAACGC22900.031.4843434
ATCAACG23200.031.0772173
TTAGCGA450.004624191730.66520911
CAACGCA24400.029.6902145
AACGCAG24800.029.2113386
TTCGGGG10350.027.7163270
CTATTAG756.14122E-527.6154651
TATTAGC1005.022921E-727.597762
AGAGTAC27400.026.44035111
GTGTATA2000.025.87377213
CGCAGAG28350.025.553488
TGTTAGA7500.024.64904870
TGTATAA2100.024.64168714
GCAGAGT29600.024.4743659
ACGCAGA30300.024.0219927
Produced by FastQC (version 0.11.5)