FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2260320
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38200.1690026190981808No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24030.10631238054788703No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA22710.10047249946910172No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21300.038.719761
GTATCAA45000.036.194611
TCGCTAA1207.6397555E-1131.64034331
TATCAAC51250.031.6235662
ATCAACG51650.031.4454233
TCAACGC52300.030.8568084
CGCTAAT1251.2369128E-1030.38145832
CAACGCA53400.030.3503325
AACGCAG53750.030.1527026
AGAGTAC59800.027.04626711
TTAACGG3400.026.41796735
CGCAGAG61650.026.400728
CAGAGTA63600.025.48451810
GCAGAGT64050.025.3581879
ACGCAGA64950.025.111887
AGTACGG52900.024.70695513
GAGTACG53500.024.42986712
TCGTATG1701.6189006E-1024.42316240
GTGGTAT11750.024.3755591
TATTAGA4050.023.8401992
Produced by FastQC (version 0.11.5)