FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757190

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757190
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences849828
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATAGTGTTCCTCCGACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT62780.738737721044729No Hit
CTATAATATTGGTGACAGTAGTAAAGTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC60710.7143798509816104No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54590.6423652786210857No Hit
ACTTAGGAGCCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGAACAAAACACT37120.4367942689579538No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35340.4158488541210692No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT34770.40914161453847137No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31800.3741933661870402No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31570.37148693618002704No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30940.3640736713782083No Hit
CTCCTAAGTTGCTCGTTTCCTGGGCCTCTACCCGGGAATCCGGGGTCCCT28930.34042182653431047No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27580.32453625910184175No Hit
TTACTACTGTCACCAATATTATAGTGTTCCTCCGACTTTTGGCCAGGGGA26290.30935671688859395No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25970.3055912490527495No Hit
GTAGTAAAGTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA25850.30417919861430787No Hit
CAGTAGTAAAGTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA25170.29617757946313844No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCTGGCCAAAAGTC25020.29441251641508637No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24770.2914707446683329No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG23840.28052735377041No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23310.2742907976672927No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22630.26628917851612327No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22600.2659361659065128No Hit
CTTTACTACTGTCACCAATATTATAGTGTTCCTCCGACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAA22380.2633474067693698No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21950.25828755936495384No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC21650.2547574332688497No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC21420.2520510032618365No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGTCAGAGTGTTTTGTT20840.24522609280936847No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20480.24098994149404349No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT20360.23957789105560184No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20180.23745981539793934No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20030.23569475234988727No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18940.2228686275340422No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC18670.21969151404754844No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18450.21710275491040543No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18130.21333728707456098No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18050.21239592011559985No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17630.20745374358105403No Hit
GTCAGGGACCCCGGATTCCCGGGTAGAGGCCCAGGAAACGAGCAACTTAGGAGCCTGTCCTGGTTTCTGCTGGTA17020.2002758205189756No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTG17000.20004047877923534No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16980.19980513703949507No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCACTTTA16930.19921678269014437No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16580.19509830224468952No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16040.18874407527170203No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCACTTTACTACTGTCACCAATATTATAGTGTT16030.1886264044018319No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGTCAGAGTGTTTTGTTCAGCTCCAA15470.18203683568910414No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15400.18121313960001317No Hit
AACCAGGACAGGCTCCTAAGTTGCTCGTTTCCTGGGCCTCTACCCGGGAA15170.178506709593No Hit
GGCTGAAGATGTGGCACTTTACTACTGTCACCAATATTATAGTGTTCCTC15150.1782713678532597No Hit
GTTTTGTTCAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA15020.17674164654494792No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14840.17462357088728545No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGTCAGAGTGTTTTGTTCAGCTCCAACAATAAGAACTA14480.17038741957196044No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGTAGAGGCCCAGGAAA14090.16579825564702505No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13560.1595616995439077No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13440.15814964910546603No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT13340.15697294040676468No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13260.15603157344780355No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13210.15544321909845288No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13130.15450185213949175No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12980.15273678909143967No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCACTTTACTACTGTCACC12940.1522661056119591No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGT12810.1507363843036473No Hit
GGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC12300.14473516994027025No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12140.14285243602234804No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG12040.14167572732364667No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA12010.14132271471403626No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC11830.13920463905637376No Hit
CCTTGGTCCCCTGGCCAAAAGTCGGAGGAACACTATAATATTGGTGACAGTAGTAAAGTGCCACATCTTCAGC11820.13908696818650362No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11660.13720423426858142No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11660.13720423426858142No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11520.13555684209039948No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTAATGA11410.13426246252182794No Hit
GGATCTCTGGTGCCTACGGGGACATCGTAATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG11390.13402712078208767No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11290.13285041208338627No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10970.12908494424754186No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10730.12626084337065854No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT10700.1259078307610481No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10510.1236720842335155No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10440.12284838814442452No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC10350.12178935031559328No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10340.12167167944572314No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10230.12037729987715161No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC10220.12025962900728147No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10200.1200242872675412No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCTGGCCAAAAGTCGGAGGAACA10180.11978894552780092No Hit
CTACGGGGACATCGTAATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG10090.11872990769896967No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGT10050.11825922421948912No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT9980.11743552813039816No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9950.11708251552078773No Hit
CCACTGAATCGGTCAGGGACCCCGGATTCCCGGGTAGAGGCCCAGGAAAC9870.11614114856182663No Hit
GGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCA9730.11449375638364469No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT9720.11437608551377455No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9630.1133170476849433No Hit
GTAATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGC9550.1123756807259822No Hit
ACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGC9490.11166965550676138No Hit
GTACCAGCAGAAACCAGGACAGGCTCCTAAGTTGCTCGTTTCCTGGGCCTCTACCCGGGAATCCGGGGTCCCTGA9450.11119897202728081No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT9400.11061061767793012No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC9390.11049294680805999No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT9310.10955157984909888No Hit
ACTTAGCTTGGTACCAGCAGAAACCAGGACAGGCTCCTAAGTTGCTCGTT9290.10931623810935859No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCA9190.10813952941065721No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9130.1074335041914364No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9110.1071981624516961No Hit
GGTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAA9030.106256795492735No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG8910.10484474505429334No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8900.1047270741844232No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC8800.10355036548572183No Hit
CCCGGGTAGAGGCCCAGGAAACGAGCAACTTAGGAGCCTGTCCTGGTTTC8800.10355036548572183No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG8650.10178530243766974No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC8630.10154996069792947No Hit
GAACAAAACACTCTGACTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA8630.10154996069792947No Hit
CTACTTAGCTTGGTACCAGCAGAAACCAGGACAGGCTCCTAAGTTGCTCGTTTCCTGGGCCTCTACCCGGGAATC8580.10096160634857877No Hit
CAATAAGAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGGCTCCTAAGTTGCTCGTTTCCTGGGCCTCTAC8510.1001379102594878No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)