FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences947830
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18920.19961385480518656No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17390.18347171961216674No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16790.17714147051686482No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15750.16616903875167488No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12440.13124716457592606No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11620.12259582414568013No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10600.11183440068366692No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10420.10993532595507632No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10240.10803625122648576No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10110.10666469725583701No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCCTGGTCCCTCCGCCGAAAATG9970.10518763913359992No Hit
GTAATATGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGAAAAATCAGTCCCAGATCCACGTCCA9740.10276104364706751No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9710.10244453119230242No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9670.10202251458594896No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC4300.050.3966371
ATCGCGT552.59206E-950.1947237
TCGCGTA352.2598899E-549.33237538
GACGTTA355.333321E-447.59679469
TTATACA4650.045.817632
TACACAT5750.036.4548955
ACGAGAC5750.035.8553822
CGAGACT5900.035.52807623
TATACAC6100.035.4898873
CACGAGA6200.035.4679721
CCCACGA5750.035.2559319
TCAACGC19400.035.248874
TCTCCGA5900.034.94380611
CGTATGC501.8284668E-434.60421441
ATCAACG20050.034.4489143
CCACGAG6000.034.35959620
CAACGCA20100.034.192265
ATCTCCG5850.034.0677210
AACGCAG20700.033.201186
TCCGAGC6350.032.46747213
Produced by FastQC (version 0.11.5)