FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757192

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757192
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences947830
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47860.5049428695019149No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16960.17893504109386704No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16380.17281580030174187No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14810.15625164850236856No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12260.1293480898473355No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11950.12607746114809618No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11450.12080225356867792No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11130.11742612071785025No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11000.11605456674720149No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9830.10371058101136281No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCCTGGTCCCTCCGCCGAAAATG9700.10233902704071406No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9490.10012343985735837No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9490.10012343985735837No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG19800.035.0226753
TCAACGC20050.034.7572024
CAACGCA20200.034.4991045
AACGCAG20350.034.248426
ACGCAGA21450.032.0169837
GTGTCGA552.9971087E-431.81077855
AGAGTAC22350.030.42846111
CGCAGAG22750.030.0444328
CGCGTAT703.9686354E-529.44052112
CAGAGTA23300.029.18781510
GTATCAA24300.028.6812931
GCAGAGT25050.027.1487469
TATCAAC25850.026.8258782
GTGGTAT3700.025.9815641
AGTACGG24400.024.77508413
ATCGGAA851.3481175E-424.59828451
GATCGGA851.3525343E-424.58643550
GAGTACG24600.024.29441512
TACGGGG14850.022.6668915
GTACGGG27450.022.2725514
Produced by FastQC (version 0.11.5)