FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1015084
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT32480.31997351943287455No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT32150.31672255695095186No Hit
GTGTATTACTGTGCGAGAGGTGGCAATTTGGGGTGGTGGCTGATTCGGGAACGATACCAGTACTACTTTGACTA26670.26273687694811465No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAACTCAGCCAATAAGCAC24780.24411772818801206No Hit
GTAATACACGGCCGTGTCTTCGGCTCTCAGGCTGTTCATCTGCAGATACA24360.2399801395746559No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC23700.23347821461081053No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC23180.2283554858514172No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA18970.18688108570325213No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT18940.186585543659441No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG18110.17840888044733244No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC16290.16047932978945584No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT16030.1579179654097592No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT15670.15437146088402537No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC15540.15309077869417703No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG15520.1528937506649696No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG15280.1505294143144804No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT15200.14974130219765064No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14930.14708142380335026No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT14800.14580074161350193No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG14710.14491411548206848No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC14650.1443230313944462No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT14400.14186018102935324No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT14360.14146612497093836No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT14200.1398899007372789No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA13870.13663893825535622No Hit
GTATTACTGTGCGAGAGGTGGCAATTTGGGGTGGTGGCTGATTCGGGAAC13670.13466865796328187No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA13510.13309243372962237No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA13210.13013701329151084No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA12850.12659050876577702No Hit
CAGTAATACACGGCCGTGTCTTCGGCTCTCAGGCTGTTCATCTGCAGATA12560.12373360234226921No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT12550.1236350883276655No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG12320.12136926599177998No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG12150.1196945277435168No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAG12080.11900492964129078No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA12080.11900492964129078No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA12040.11861087358287589No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC11880.11703464934921642No Hit
GTATTACTGTCAGCAGTATGGTAGCTCACCTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA11450.11279854672125657No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG11340.11171489256061568No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC11300.11132083650220079No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTCCACCCTGGGG11290.11122232248759707No Hit
GTGGGCATGTGTGAGTTTTGTCACAAGATTTGGGCTCAACTTTCTTGTCC11230.11063123839997477No Hit
GGGCTGGAGTGGGTGGCCAACATAAAGCAAGATGGAAGTGAAGAATATTA11120.10954758423933389No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA11070.1090550141663153No Hit
CTCCAGAGACAACGCCAAGAACTCACTGTATCTGCAGATGAACAGCCTGAGAGCCGAAGACACGGCCGTGTATTA10960.10797136000567441No Hit
GTACTACTTTGACTACTGGGGCCAGGGAACCCTGGTTACCGTCTCCTCAGCCTCCACCAAGGGCCCATCGGTCTT10680.10521296759677032No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC10420.10265160321707367No Hit
CTCCACGGCGATGTCGCTGGGATAGAAGCCTTTGACCAGGCAGGTCAGGCTGACCTGGTTCTTGGTCAGCTCATC10390.1023560611732625No Hit
GTCTCAGAGAGGAGCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCA10370.10215903314405507No Hit
GAGTTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGT10290.10137092102722534No Hit
GAGGAAGACTGACGGTCCCCCCAGGAGTTCAGGTGCTGGGCACGGTGGGC10240.10087835095420675No Hit
GCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGGTTTTC10200.10048429489579187No Hit
ACCCTGGGGGGTCCCTGAGACTCTCCTGTGAAACGTCTGGATTCTCCTTT10200.10048429489579187No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTAG306.1953394E-446.17312242
CGATTAA650.00121671240.25393370
GTATCAA15600.040.0191571
TTAAATG700.001751268937.37865470
TATCAAC17850.034.969562
ATCAACG18150.034.201543
TCAACGC18400.033.7368434
CAACGCA18600.033.3740845
AACGCAG18950.032.764136
AGAGTAC19450.031.23095711
CGTTAGC450.004525100330.80036443
CGCAGAG20850.029.6261318
GCAGAGT21350.028.936599
CAGAGTA20900.028.89907610
GTGGTAT4150.028.2582171
TTGGACC1005.033853E-727.5892414
CAGCGCT4850.026.309334
GTGTATA1057.698309E-726.2793521
TACGGGT2650.026.04426415
TTAACGG1208.350435E-825.92749635
Produced by FastQC (version 0.11.5)