FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1026001
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28000.042.4445151
GGTATCA11700.036.5086981
TCAACGC33050.035.7418754
TATCAAC33200.035.580392
CAACGCA33500.035.5701685
ATCAACG33600.035.259313
AACGCAG34200.035.0435266
GTGGTAT4100.034.447721
ACGCAGA36750.032.6230667
CGCAGAG37550.032.1240588
AGAGTAC37550.031.84870711
CAGAGTA38500.031.06282810
GCAGAGT41400.028.9701759
AGTACGG33100.026.96770513
TTAACGG2700.026.86469335
GAGTACG33500.026.74858312
TGGTATC5850.024.7256722
TAACGGC3000.024.1805936
TACGGGT4250.023.51696815
GTACGGG38050.023.45942514
Produced by FastQC (version 0.11.5)