FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences597542
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15450.040.8134161
GGTATCA6100.038.9762081
TCAACGC17350.036.5333634
CAACGCA17750.035.9041525
AACGCAG17950.035.696026
ATCAACG17850.035.5100173
TATCAAC18300.034.8250662
ACGCAGA19200.033.372067
CGCAGAG20250.031.6416578
AGAGTAC20400.031.24274411
CAGAGTA20800.030.64192410
GCAGAGT21900.029.2576969
TGATCGA500.00778529727.56116910
AGTACGG18900.026.79558213
GAGTACG19050.026.58459312
GTGGTAT2750.026.3127941
AATAGCG1508.3491614E-1025.78445462
TAATCGT1251.23684E-724.89254838
TTAATCG1301.8128776E-723.92710337
TGGTATC2950.023.3549672
Produced by FastQC (version 0.11.5)