FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757201

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757201
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1434806
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC87770.6117203301352239No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT74420.5186763924879043No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC63610.44333519653528075No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC61190.4264688048419089No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA59560.41510838399058825No Hit
GCCTTGGGCTGACCGAGGACGGCGACCTTGGTCCCAGTTCCGAAGACGTGACCATTCAGGCTGTCATCCCATG57900.40353887563893653No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG50120.34931551721974957No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC46200.321994750509825No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC45880.31976448383962713No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC45060.3140494254972449No Hit
GTAATAATCACCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGC43010.29976177964128947No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG41870.29181645462870937No Hit
GATTATTACTGTTCAGCATGGGATGACAGCCTGAATGGTCACGTCTTCGG41620.2900740587926173No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT40590.2828953879479177No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT38790.27035013792805435No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG37570.2618472462479248No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT37380.2605230254124948No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT36720.2559231004052116No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCGAGGACGGCGACCTTGGTC33500.23348104203634498No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC32870.22909020452939283No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC32780.22846294202839967No Hit
GTCTGAGGATGAGGGTGATTATTACTGTTCAGCATGGGATGACAGCCTGAATGGTCACGTCTTCGGAACTGGGAC32440.22609328369131437No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGCCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC31680.2207964003495943No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG29610.2063693628267515No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC29000.2021179169866867No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC28410.1980058628135093No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGGCTCCCTGGCCATCAGTGGGCTCCAGT27620.19249989197145817No Hit
CCCCTGAGGGCCGCTGACTATTAGTAAAGATGAGGAGTTTGGGGGCCGTT26880.18734240029662547No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG26760.18650605029530126No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC25970.1810000794532501No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT25940.1807909919529191No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG25620.17856072528272116No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA25530.17793346278172797No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT25260.1760516752787485No Hit
GGATGACAGCCTGAATGGTCACGTCTTCGGAACTGGGACCAAGGTCGCCGTCCTCGGTCAGCCCAAGGCCAACCC24650.1718002294386837No Hit
GCATGGGATGACAGCCTGAATGGTCACGTCTTCGGAACTGGGACCAAGGT24240.1689427002674926No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGGTGATTATTACTGTTCAGCATGGGATGACAGCCTGAATGGTCA23950.16692152109762576No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG23360.16280946692444834No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA22720.1583489335840525No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC22490.15674592941484772No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA22430.15632775441418562No Hit
CTGATGGCCAGGGAGCCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC22150.15437627107776242No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA22140.15430657524431876No Hit
GTCTGGCACCTCAGGCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG21870.15242478774133925No Hit
CCTCAGGCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATGAGGGTGAT21700.1512399585727966No Hit
ATCCCATGCTGAACAGTAATAATCACCCTCATCCTCAGACTGGAGCCCAC21570.15033391273802868No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGG21320.1485915169019366No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG20570.1433643293936602No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20570.1433643293936602No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT20550.1432249377267728No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC20430.1423885877254486No Hit
GACCGAGGACGGCGACCTTGGTCCCAGTTCCGAAGACGTGACCATTCAGG20410.14224919605856123No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG20410.14224919605856123No Hit
GTTCAGCATGGGATGACAGCCTGAATGGTCACGTCTTCGGAACTGGGACC20370.1419704127247865No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG20360.14190071689134282No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT20290.141412846057237No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT20140.14036740855558175No Hit
GAACAGTAATAATCACCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGCCTGAGGTGCCAGACTTGGAG19850.13834622938571486No Hit
CTATTAGTAAAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTGCTGGTA19790.13792805438505276No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG19700.13730079188405958No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG19600.13660383354962274No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG19480.1357674835482985No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG19200.13381600021187534No Hit
TTACTGTTCAGCATGGGATGACAGCCTGAATGGTCACGTCTTCGGAACTG19060.13284025854366374No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG19000.13242208354300164No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT18810.13109786270757162No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC18540.12921607520459213No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC18510.12900698770426106No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC18420.1283797252032679No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA18160.1265676335337321No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA17970.12524341269830205No Hit
CTTCGGAACTGGGACCAAGGTCGCCGTCCTCGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC17910.12482523769763995No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC17840.12433736686353417No Hit
ATGCTGAACAGTAATAATCACCCTCATCCTCAGACTGGAGCCCACTGATG17700.12336162519532258No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT17480.1218283168595615No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG17410.12134044602545571No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC17370.12106166269168095No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG16960.11820413352048988No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA16840.11736778351916566No Hit
GTTCCGAAGACGTGACCATTCAGGCTGTCATCCCATGCTGAACAGTAATAATCACCCTCATCCTCAGACTGGAGC16810.11715869601883461No Hit
GTCCTCGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC16700.11639204185095407No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC16640.11597386685029194No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG16630.11590417101684829No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT16630.11590417101684829No Hit
GACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGG16480.114858733515193No Hit
CCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT16480.114858733515193No Hit
CCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACA16350.11395268768042507No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG16340.11388299184698139No Hit
CTATAAACTGGTACCAGCAACTCCCAGGAACGGCCCCCAAACTCCTCATC16010.1115830293433398No Hit
CTCTTGTTCTGGAAGCAGTTCCAACATCGGAAGTAATACTATAAACTGGTACCAGCAACTCCCAGGAACGGCCCC15930.11102546267579032No Hit
CCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAG15920.11095576684234662No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTG15910.11088607100890294No Hit
GCCCCCAAACTCCTCATCTTTACTAATAGTCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT15860.11053759184168452No Hit
CTGTCATCCCATGCTGAACAGTAATAATCACCCTCATCCTCAGACTGGAG15860.11053759184168452No Hit
GTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTT15710.10949215434002925No Hit
GACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGG15610.10879519600559238No Hit
GACTGGAGCCCACTGATGGCCAGGGAGCCTGAGGTGCCAGACTTGGAGCC15520.10816793350459923No Hit
GTCATCCCATGCTGAACAGTAATAATCACCCTCATCCTCAGACTGGAGCC15390.10726188766983132No Hit
GTCAGGGACCCCTGAGGGCCGCTGACTATTAGTAAAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTGCTGGTA15360.10705280016950026No Hit
GTTTGGGGGCCGTTCCTGGGAGTTGCTGGTACCAGTTTATAGTATTACTT15260.10635584183506341No Hit
ACTATAAACTGGTACCAGCAACTCCCAGGAACGGCCCCCAAACTCCTCAT15250.10628614600161973No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG15170.10572857933407025No Hit
CTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC15150.1055891876671829No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGGCTCCCTGGC15130.10544979600029551No Hit
TTACTAATAGTCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGGCTCCCTGG15100.10524070849996445No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG15040.10482253349930234No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA14900.10384679183109075No Hit
GGATGAGGGTGATTATTACTGTTCAGCATGGGATGACAGCCTGAATGGTCACGTCTTCGGAACTGGGACCAAGGT14880.10370740016420338No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC14720.10259226682910444No Hit
GGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGT14690.10238317932877337No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA14610.10182561266122389No Hit
TCCTCGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG14540.10133774182711809No Hit
GGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC14500.10105895849334336No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG14500.10105895849334336No Hit
GCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCA14390.10029230432546281No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)