FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1434806
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC73310.5109401549756553No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC70140.4888465757740071No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT67580.4710044424124237No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC59120.41204176731906617No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC56850.39622081312734964No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA48640.33900053387008416No Hit
GCCTTGGGCTGACCGAGGACGGCGACCTTGGTCCCAGTTCCGAAGACGTGACCATTCAGGCTGTCATCCCATG46550.324434104680354No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC46050.32094931300816976No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT45860.31962509217273977No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG44630.3110525046591665No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG43330.3019920463114874No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC42900.29899512547340895No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41700.29063162546016674No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT41220.2872862254548698No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC41120.286589267120433No Hit
GATTATTACTGTTCAGCATGGGATGACAGCCTGAATGGTCACGTCTTCGG39720.2768318504383171No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT35600.24811716705951886No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG35380.24658385872375777No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC34520.24059001704760086No Hit
GTCTGAGGATGAGGGTGATTATTACTGTTCAGCATGGGATGACAGCCTGAATGGTCACGTCTTCGGAACTGGGAC33790.23550222120621184No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT33530.23369012953667603No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG31150.21710252117707898No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG31100.21675404200986056No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC29360.20462696699065935No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC28520.1987725169813898No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGGCTCCCTGGCCATCAGTGGGCTCCAGT28430.19814525448039666No Hit
CCCCTGAGGGCCGCTGACTATTAGTAAAGATGAGGAGTTTGGGGGCCGTT27800.19375441697344448No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCGAGGACGGCGACCTTGGTC27600.1923605003045708No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT26680.18594848362775176No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT25650.1787698127830522No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA24940.17382140860855055No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC24920.17368201694166321No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA24550.17110327110424686No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG23990.16720030443140046No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC23980.16713060859795678No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT23740.16545790859530837No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA23680.16503973359464624No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG23550.16413368775987833No Hit
GGATGACAGCCTGAATGGTCACGTCTTCGGAACTGGGACCAAGGTCGCCGTCCTCGGTCAGCCCAAGGCCAACCC23320.16253068359067357No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGGTGATTATTACTGTTCAGCATGGGATGACAGCCTGAATGGTCA22960.1600216335867009No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT22350.15577018774663615No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT22340.15570049191319246No Hit
GTCTGGCACCTCAGGCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG22190.15465505441153718No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC21660.15096117523902186No Hit
CTCTTGTTCTGGAAGCAGTTCCAACATCGGAAGTAATACTATAAACTGGTACCAGCAACTCCCAGGAACGGCCCC21480.14970665023703553No Hit
GCATGGGATGACAGCCTGAATGGTCACGTCTTCGGAACTGGGACCAAGGT21420.14928847523637342No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA21280.14831273356816183No Hit
CTGATGGCCAGGGAGCCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC20900.1456642918973018No Hit
CCTCAGGCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATGAGGGTGAT20480.14273706689266702No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC20070.13987953772147593No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC19990.13932197105392646No Hit
GTTCAGCATGGGATGACAGCCTGAATGGTCACGTCTTCGGAACTGGGACC19820.1381371418853838No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT19650.13695231271684116No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC19540.13618565854896064No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG19470.13569778771485483No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA19470.13569778771485483No Hit
GTAATAATCACCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGC19370.13500082938041796No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG19260.13423417521253744No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG19260.13423417521253744No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG19130.13332812937776953No Hit
TTACTGTTCAGCATGGGATGACAGCCTGAATGGTCACGTCTTCGGAACTG18630.1298433377055853No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG18620.12977364187214158No Hit
GGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG18510.12900698770426106No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG18490.1288675960373737No Hit
CTATTAGTAAAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTGCTGGTA18360.1279615502026058No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT18330.12775246270227472No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG18240.12712520020128157No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT18130.12635854603340102No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAGTTCCAACATCGGAAGTAATACT18080.1260100668661826No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG17970.12524341269830205No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA17870.12454645436386523No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA17610.1227343626943294No Hit
CTATAAACTGGTACCAGCAACTCCCAGGAACGGCCCCCAAACTCCTCATC17580.12252527519399836No Hit
GTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGG17210.11994652935658201No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG17070.11897078768837042No Hit
GCCCCCAAACTCCTCATCTTTACTAATAGTCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT16890.11771626268638408No Hit
GTTTGGGGGCCGTTCCTGGGAGTTGCTGGTACCAGTTTATAGTATTACTT16840.11736778351916566No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC16820.11722839185227829No Hit
ACTATAAACTGGTACCAGCAACTCCCAGGAACGGCCCCCAAACTCCTCAT16780.11694960851850354No Hit
CTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC16650.11604356268373564No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC16570.11548599601618616No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG16540.1152769085158551No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT16430.11451025434797456No Hit
GTTCCAACATCGGAAGTAATACTATAAACTGGTACCAGCAACTCCCAGGAACGGCCCCCAAACTCCTCATCTTTA16320.11374360018009404No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTG16310.11367390434665035No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC16240.11318603351254455No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC16060.11193150851055822No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG15960.11123455017612138No Hit
GTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGTTCCAACATCGGAAGTAATACTATAAA15860.11053759184168452No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGC15850.11046789600824083No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT15740.10970124184036031No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG15670.10921337100625451No Hit
TTACTAATAGTCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGGCTCCCTGG15650.10907397933936713No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC15640.10900428350592345No Hit
CCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT15520.10816793350459923No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG15510.10809823767115555No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGGCTCCCTGGC15500.10802854183771186No Hit
CTTCGGAACTGGGACCAAGGTCGCCGTCCTCGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC15270.1064255376685071No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG15150.1055891876671829No Hit
GACCGAGGACGGCGACCTTGGTCCCAGTTCCGAAGACGTGACCATTCAGG15050.10489222933274603No Hit
CCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAG14980.10440435849864023No Hit
GAAGTAATACTATAAACTGGTACCAGCAACTCCCAGGAACGGCCCCCAAA14920.10398618349797813No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG14720.10259226682910444No Hit
GGATGAGGGTGATTATTACTGTTCAGCATGGGATGACAGCCTGAATGGTCACGTCTTCGGAACTGGGACCAAGGT14680.10231348349532968No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC14680.10231348349532968No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC14640.10203470016155496No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGG14620.10189530849466757No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA14580.10161652516089283No Hit
CTCCTCATCTTTACTAATAGTCAGCGGCCCTCAGGGGTCCCTGACCGATT14510.10112865432678703No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)