FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1372443
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18710.13632624451434414No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC16090.1172361985160768No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA74400.043.394651
GGTATCA26750.041.3405461
TATCAAC89100.036.0405122
TCAACGC88850.035.9877974
ATCAACG89850.035.7777753
CAACGCA91400.035.1335835
AACGCAG91600.035.099366
GTGGTAT9950.035.0973661
ACGCAGA95300.033.7848747
AGAGTAC97400.033.0952411
TGGTATC10750.032.8015672
CAGAGTA98200.032.72097410
CGCAGAG98400.032.6544658
GCAGAGT102650.031.3024799
AGTACGG74350.030.91551213
GAGTACG75100.030.56115312
GTACGGG80200.028.40417514
TACGGGG28200.027.2103315
TACGGGT23550.027.20104615
ACGGGTA8750.026.61684616
Produced by FastQC (version 0.11.5)