FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1610401
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGGGACAGACTTCACTCTCACCATCACCAGCCTTGAGCCTGAAGATT53350.331283947290147No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46630.289555210161941No Hit
GTCCCAGACCGACTGCCACTGAACCTGGCAGGGATGCCAGTGGCCCTGTT40460.2512417714594067No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA39570.2457151976433199No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32160.19970181339927137No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTC29270.1817559725807423No Hit
GTCTCCAGGCACCCTGTCGTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAACAG28980.1799551788653882No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27490.1707028249485687No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26840.16666656317277498No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAACAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT25570.15878032862622415No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23990.14896910769429478No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23930.1485965296842215No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23590.14648525429380632No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22790.14151754749282944No Hit
GTTCAGTGGCAGTCGGTCTGGGACAGACTTCACTCTCACCATCACCAGCC22770.141393354822805No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22650.14064819880265847No Hit
CTCCAGGCACCCTGTCGTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC19690.12226768363904393No Hit
GACTTCACTCTCACCATCACCAGCCTTGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTGTCAACTGG19440.12071527526373867No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC19410.12052898625870202No Hit
CACCATCACCAGCCTTGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGC19050.11829351819826242No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18940.1176104585131281No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18580.1153749904526885No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT18250.11332581139728554No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18120.11251855904212678No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17240.10705408156105219No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCTGCCAGGTTCAGTGGCAGTCGGTCTGGGACAGACTTCACT16860.10469442083058816No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16610.10314201245528287No Hit
GTAATATGTGGCTGTGTCCACAGGGTCCATGTTGGTCATTGTAAGGACCA16280.1010928333998799No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC16150.10028558104472116No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)