FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1789501
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37770.21106442522245025No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG25170.14065373531504036No Hit
GTATATTACTGTGCGAAAGACTGGGGCGCTGGCCACATCTGGGCAGTTCGTCACCACTACAACGGTATGGACGT24960.1394802238165835No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT24250.13551263732180088No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23910.13361266632429936No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23650.13215974732620994No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23440.1309862358277531No Hit
GATCTATGCTGCATCCAGTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA23080.12897450183039855No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22300.12461574483613029No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21730.1214304993403189No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC21590.12064815834134768No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21360.11936288384303781No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21100.11790996484494838No Hit
CTGTTAGCCTGTTGACAATAGTAAGTTGCAACATCTTCAGGCTGCAGGCTGCTGATAGTGAGAGTGAAATCTGTC21080.11779820184509536No Hit
GTAATATACAGCCGTGTCTTCAGCTCTCAGGCTGTTCATTTGCAGATACA20700.11567470484788776No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20030.1119306443528112No Hit
GGCTAACAGTTTCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT19360.10818658385773465No Hit
CTGTAGGAGACACAGTCACCATCACTTGTCGGGCGAGTCAGGATATTAGC19230.10746012435868994No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC18800.10505721986184975No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG18740.10472193086229066No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18100.10114551486699365No Hit
CACTATCAGCAGCCTGCAGCCTGAAGATGTTGCAACTTACTATTGTCAACAGGCTAACAGTTTCCCGCTCACTTT18030.10075434436750805No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34150.041.05181
GTGGTAT6650.036.2580871
TATCAAC39650.035.263582
ATCAACG41200.033.5189743
CAACGCA41400.033.4402285
TCAACGC41300.033.4378134
AACGCAG42200.032.8952526
AGAGTAC46200.030.35898811
CAGAGTA46750.030.00098410
ACGCAGA47300.029.3557937
CGCAGAG48100.029.0140518
GCAGAGT48850.028.5701929
AGTACGG40800.027.197613
GAGTACG41550.026.4578512
TAACCCG809.5401687E-426.10115869
TACGGGG21000.024.45402715
GTACGGG46100.023.84649814
GAGTACT13650.022.46943712
TACGGGA19050.021.52957315
AGTACTT13950.021.49215113
Produced by FastQC (version 0.11.5)