FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1424864
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCCTCAGGTCTCAGGCTGTTCATTTGCAGATAGA68580.48130909335908545No Hit
GTCTATTACTGTGCGAAAGGCCGGTGGGAACTAGTAGATGCTTGTGATAT53100.3726671457767197No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39950.28037763604105376No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT38810.27237687245940667No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT37780.2651481123812518No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT36720.25770880589305367No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC35820.2513924135917533No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG34340.24100545736294834No Hit
GGGTATTAGCAGGTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAGTCTCCTGATCTATGCTGCATC31850.22353010532935075No Hit
GATCTATGCTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA31680.2223370090057718No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29430.2065460282525209No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29140.2045107462887686No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG28270.1984049003975116No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCCATATTCAT28010.19658016484380264No Hit
GTACTATGCAGACTCCGTGAAGGGCCGCTTCACCATCTCCAGAGACAATT27750.19475542929009365No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26040.18275428391762302No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC25850.18142082332068182No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC25530.17917499494688616No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24310.17061277427179014No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG24290.17047240999842792No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23930.16794585307790777No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23900.16773530666786446No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23840.16731421384777775No Hit
CTGTTAGCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATAGTGAGAGTGAAATCTGTC23760.16675275675432885No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC23720.16647202820760437No Hit
GATAAGTACTATGCAGACTCCGTGAAGGGCCGCTTCACCATCTCCAGAGACAATTCCAAGAACACGCTCTATCTG23610.1657000247041121No Hit
CACTATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGCTAACAGTTTCCCGCTCACCTT23220.16296292137354865No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA22870.16050654658970961No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC22870.16050654658970961No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA22410.1572781683023783No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC21920.1538392436050037No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC21720.15243560087138142No Hit
GCATAGATCAGGAGACTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC21630.15180396164125137No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT21600.15159341523120803No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT21580.1514530509578458No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT21320.14962831540413682No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG21250.149137040447369No Hit
GGCTAACAGTTTCCCGCTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT20980.14724212275697893No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAGCAGGTGGTTAGCCTGGTATCAGCAGAAA20760.1456981157499944No Hit
CTCCTGTGCAGCCTCTGGATTCAACTTCAATGAATATGGCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGG20650.14492611224650212No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19960.1400835448155052No Hit
CTCCTGATCTATGCTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTT19480.1367148022548117No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA19300.13545152379455164No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG19290.1353813416578705No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19190.13467952029105934No Hit
GTACTTATCAGTTCCATCATATGATGTAACTGCCACCCACTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTG19110.13411806319761044No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18410.1292053136299324No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGG18240.12801221730635345No Hit
GAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGTTGCTCTTTTAAGAGGTGTCCAGTGT17480.12267837491858871No Hit
GCACTAGAAGTCGGCGGTGTTTCCATTCGGTGATCAGCACTGAACACAGA17230.12092382150156085No Hit
GCATAGTACTTATCAGTTCCATCATATGATGTAACTGCCACCCACTCCAGCCCCTTGCCTGGAGCCTGGCGGACC16990.11923945022121409No Hit
GGGCTGGAGTGGGTGGCAGTTACATCATATGATGGAACTGATAAGTACTA16870.11839726458104073No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT16600.11650234689065062No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16450.11544961484043391No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT16450.11544961484043391No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG16440.11537943270375278No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16340.11467761133694163No Hit
GCCTGAGACCTGAGGACACGGCTGTCTATTACTGTGCGAAAGGCCGGTGGGAACTAGTAGATGCTTGTGATAT16190.11362487928672492No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG15950.11194050800637816No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT15850.111238686639567No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGA15810.11095795809284255No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15760.11060704740943697No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTGAGCGGGAAA15630.10969467963258249No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG15180.10653648348193231No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14930.10478193006490445No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14760.10358883374132549No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14730.10337828733128214No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC14710.10323792305791991No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT14690.10309755878455769No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT14440.10134300536752983No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC14430.1012728232308487No Hit
CCATTGTCCCTTGGCCCCAGATATCACAAGCATCTACTAGTTCCCACCGG14300.10036045545399422No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14270.10014990904395087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG506.773371E-848.4554540
GTATATA404.9763425E-543.163521
TCTGGAG22650.040.7074370
GTGGTAT2900.040.4844061
GTATCAA17600.040.416751
CGTATGC602.83404E-740.3880741
TATCAAC20750.034.7560272
TATATAG400.002594115734.506582
ACTAGAC400.002594115734.506583
ATCAACG21500.033.8645973
TCAACGC21800.033.5568584
CAACGCA22400.032.6580165
TATGCCG851.16609954E-732.60378343
ATGTGTA1500.032.20614211
AACGCAG23350.031.4770956
TATACAC1101.0568328E-931.3696173
CGCAGAG24450.030.0598938
AGAGTAC24150.030.00572411
AGAGCGT18750.029.8001148
TAGAGCG18800.029.52895547
Produced by FastQC (version 0.11.5)